Abstract: Three functional bacteria improving disease resistance and adversity resistance of turfgrass were isolated and chosen in the lab. The authors studied their culture conditions and physiological characteristics. The results indicated that the best N source and C source for three bacteria　(E, F and H) were peptone and sucrose respectively, beef extract and glucose second; three bacteria could grow at a pH range of 6～10, of which the bacteria E and F grew best when the initial pH value was 7.4 and bacterium H at pH 7.6;the most suitable temperature for them to grow was 30～35 ℃; the dry weight of three bacteria had a significant variance at different shaker speed and different solution amount when cultured in flasks.