• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

2007 Vol. 20, No. 6

Display Method:
Optim iza tion for SSR-anchored PCR Reaction Systemin Endangered Plant Taxus yunnanensis
MIAO Ying-chun, SU Jian-rong, ZHANG Zhi-jun
2007, 20(6): 739-743.
Abstract:
The interaction among different factorswas usually ignored during experimental design with orthogonal design which waswidely used in op timizing for PCR reaction system. Orthogonal design L64 (421 ) involving interactionwas used to op timize simp le sequence repeated-anchored PCR by degenerate p rimer ( SSRanchored PCR) amp lification system on T. yunnanensis in five factors: Taq DNA polymerase, Mg2+ , dNTP,DNA and p rimer at four levels.The analytic results by software SAS showed that: ①Three factors (Taq DNA polymerase, Mg2+ , dNTP) and the twointeractions ( Taq ×Mg2+ , Mg2+ ×dNTP) had notable effect on the SSR2anchored PCR amp lification system; ②Anop timal SSR-anchored PCR reaction system was established containing 1 ×PCR buffer, 4 U Taq DNA polymerase,2. 0 mmol·L-1Mg2+ , 0. 4 mmol·L-1 dNTP, 75 ngDNA, 1. 0μmol·L-1 p rimer in a total volume of 20μL reac2tion system. Moreover the op timal annealing temperature (54 ℃) was p roposed by gradient PCR. Orthogonal designinvolving interaction is of special app lication value as it can not only screen out the best PCR reaction system quicklyand effectively, but also reveal the extent to which the testing factors and their interaction can affect the PCR amp lification, and thusmake the analytic resultmore objective and accurate. While, further research of the judgmentalstandard to the electrophoretogram of PCR amp lification p roducts is still needed to imp rove both the facility of themethod and the reliability of experimental data.
Effects of Land-use Change on Vertica l D istr ibution and Storageof So ilOrgan ic Carbon in North Subtropica l Area s
LI Zheng-cai, XU De-ying, FU Mao-yi, SUN Xue-zhong, XI Jin-rong
2007, 20(6): 744-749.
Abstract:
This paper dealswith the effects of land2use change on the vertical distribution and storage of soil organiccarbon ( SOC) in north subtrop ical areas bymeans of field survey. The results showed that: (1) The contents of SOCof the top soil p rofile (0~10 cm) were the greatest, compared with the other soil p rofiles ( 10~100 cm) in theeight land2use types excep t the tea garden, which showed an increase from 0 to 30 cm and a decrease from 30 to 100cm in dep th. (2)When the natural secondary stands were changed into arable land, the average SOC content decreased by 21. 1% , butwhen changed into the Phyllostachys praecox stands, the average SOC content decreased by48. 5%. In the one meter soil p rofile, the contents of SOC from the highest to the lowestwas ranked as follows: teagarden, shrub, the natural secondary stands, extensive Phyllostachys edu lis stands, intensive Phyllostachys edu lisstands, masson p ines, arable land, Chinese fir, Plyllostachys praecox stands. (3) The vertical distribution of SOC storage showed the same tendency as the SOC content; after the natural secondary standswas converted into arableland, the SOC storage decreased by 22. 5% , while converted into Phyllostachys praecox stands, the SOC storage decreased by 51. 4%; In the one meter soil p rofile, the storage of SOC from highest to lowestwas ranked as follows:tea garden, shrub, the natural secondary stands, extensive Phyllostachys edulis stands, masson p ines, arable land,intensive Phy llostachys edu lis stands, Chinese fir and Phy llostachys praecox stands.
Fruit Quality of Zizyphus ju jubeM ill. cv.‘Dongzao’Influenced by Differen t Kinds of Fertilizer
HU Xin-yan, WANG Gui-xi, LIANG Li-song, XU Jiu-ru
2007, 20(6): 750-754.
Abstract:
In order to imp rove the quality of‘Dongzao’jujube, the trees were fertilized by amino acid fertilizer,mineral fertilizer and rare earth fertilizer during fruit development. The experiment showed that all the fertilizer treatments could imp rove transverse growth, single fruit weight and total sugar content of fruit, the mineral fertilizertreatment was the best. The fast increasing period of total sugar contentwas 70~90 days after blossom, and disaccharide was the main composition of the sugar. The quantity of fructose and glucose were fast increased during 90~110 days after blossom, amino acid fertilizer and rare earth fertilizer were effective for imp roving this two kinds ofsugars. Both amino acid fertilizer and rare earth fertilizer could increase Vc content of‘Dongzao’jujube fruit, whilerare earth fertilizer treatment decreased it. Pericarp of‘Dongzao’jujube fruit nourished bymineral fertilizer and amino acid fertilizerwere thinner than by rare earth fertilizer. The conclusion of this experimentwas that the effectiveness of three fertilizers to imp rove the fruit quality is mineral fertilizer better than amino acid fertilizer better thanrare earth fertilizer.
A Composition Analysis of Seed P lant Flora in Thuja sutchuenensis Community
WANG Xiang-fu, GUO Quan-shui, LIU Zheng-yu, REN Ming-bo, BAH AR-Guli, LUO Zheng-jun
2007, 20(6): 755-762.
Abstract:
The seed plants floral composition and characterist ics in Thuja su tchuenensis commun ity w ere analyzed,based on the invest igation o f p lants in Thuja sutchuenensis commun ity by sample p lo t investigation method. The resu lt show ed that therew ere 73 fam ilies of seed plants inc lud ing 150 genera and 242 species in Thuja sutchuenensiscommunity. The dom inant fam ilies o f the commun ity arem ainly of the fo llow ing: Betulaceae, Viburnaceae and Anacardiaceae. The rate of sing le-species fam ilies and themonotypic generaw as h igh. The geographic element of the flora w asmu ltiplicity , and predom inant w ith temperate florist ic e lem en.t The flora composit ion of the community reflected significan t characterist ics o f transition from Sub trop ica l flora toW arm Temperate zone. The result of flora index show ed that the types ofW idespread, N. Temp. and E. A sia& N. Amer. d isjuncted distributions ex treme ly influenced the characterist ic ofThuja sutchuenensis community.
The Impact of Flower Certif ica tion on the Developmentof Chinese Flora l Industry
WANG Yan, WU Dan
2007, 20(6): 763-767.
Abstract:
The floral industry has become one of Chinese most dynamic and fastest-growing industries, meantime ithas become a new point of economic growth. The flower certification impacted on floral industry in the direction ofecology, economy and society. In the paper the flower certification’s development in China was introduced, and itsinfluence on the flower p roduction, flower trade, the change of the industry restructure framework and the reform ofmanagement and operationalmechanism were discussed. It is deemed that the accep tance of flower certification andestablishing the flower certification system will imp rove the competition ability of Chinese flora industry in the world,and also will p romote the Chinese flora industry development in a health and sustainable way.
Study on Vertica l Structue of Forest Commun ities in Yunmengshan
ZHENG Jing-ming, ZHANG Chun-yu, ZHOU J in-xing, ZHAO Xiu-hai, YU Xin-xiao, QIN Yong-sheng
2007, 20(6): 768-774.
Abstract:
A stratification method of forest vertical structure was built based on crown cut-off height point (CCHP) instand. Compared to the result by systematic clustering of tree height in the stand, stratification by the new methodwas validated, during the p rocess of stratification of stand, tree height near the CCHP was easy to be classifiedwrongly, and the best classification results came from the p rocesswith 0. 4 of competition coefficient used, in whichthe average rate of mis-classified trees was 10. 10% in all nine p lots. Besides the number of canopy layers (Sn ) ,several other indices related with vertical structure were also calculated, i. e. , Gini index (Gc ) , variants of treeheights (Cv ) , and biodiversity index of tree layer (Ht ) , shrub layer (Hs ) and grass layer (Hg ). The correlation analysis between those indices showed that there were common correlation existed among them, which indicated that Sn ,Gc , Cv could be used to describe the vertical structure of forest stand p roperly and the comp lexity of vertical structurecould influence the development of shrub and grass layer.
Phenotypic Variation of Geographic Provenance in Tsuga dumosa
LI Li, LI Kun, CUI Kai
2007, 20(6): 768-774.
Abstract:
9 provenances of Tsuga dumosa(D.Don) Eichler were collected from 5 distribution areas in Yunnan. The quantitative trait variation of different provenances was studied and provenance test was carried out. The variance analysis showed that there were significant differences in the 12 quantitative traits among the provenances. It was found that geograph, water and temperature were the main factors affecting the morphological differentiation by using the method of principal component analysis. Correlation analysis indicated that geographic variation of Tsuga dumosa (D.Don) Eichler was continuous by altitude gradient, and the variation was random by latitude factor. After three-year cultivation, there were distinct differences in growth characteristics among 9 provenances. 4 superior provenances were selected according to the standard height of 3-year-old seedling.
Breeding System and Pollina tion Ecology in J a tropha cu rcas
LI Kun, YIN Wei-lun, LUO Chang-wei
2007, 20(6): 775-781.
Abstract:
Plant flowering and breeding characteristics are important to understand rep roduction of p lant population.Flowering characteristics and pollination ecology of Jatrapha curcas were investigated in Yuanjiang County, YunnanProvince. The breeding system, pollen-ovule ratio ( P /O) and pollen viability were determined. The results were asfollows: (1) Jatropha curcas wasmonoecious, The p lant p roduced flowers in dichasial inflorescences. Normally, theflowers were unisexual, and male and female flowerswere p roduced in the same inflorescence. In a few, onlymaleflowerswere p roduced in an inflorescence, and fruitwere p roduced only through pollination between different flowersfrom the same or different p lant. The 1 ife span of the male flowerwas about 2 days. The life span of the female flower was about 5~12 days. The diameter of female flowerwas bigger than that of the male flower slightly. (2) The pollen viability ofmale flowerwas relatively high in 9 hours after blooming and get low 33 hours later. The pollen hardly had any viability after 48 hours. The stigmatic recep tivity of female flowerwas strong during 1st -4th day and beganto decline on 5th day. On 9th day, stigma comp letely lost its recep tivity. (3) The effective pollination way of J. curcas was insect pollination. (4) J. curcas was out-crossing, partly self-compatible, requiring pollinator.
Studies on Al ka line Solution Extraction of Polysacchar idefrom Sil kworm Pupa and Its Immunomodula ting Activ ities
SUN Long, FENG Ying, HE Zhao, MA Tao, ZHANG Xin
2007, 20(6): 782-786.
Abstract:
The extraction of polysaccharide from silkworm pupa by alkaline solution was studied. The op timum conditionswere obtained by using orthogonal design L9 (34 ). The result showed the op timum conditions were as follows: concentration of alkaline solution 0. 02 mol·L-1 , temperature 80 ℃, time 3 hours. Under these conditions,the contents of total polysaccharide, p rotein and amino acids in extractiveswere 27. 9% , 38. 1% and 35. 85% respectively. The immunological test in mice showed the polysaccharide of silkworm pupa ( PSP) could increase significantly macrophage phagocytosis, enhance remarkably hemolysin antibody and lymphocyte transformation, whichindicated PSP had evident nonspecific, cellular and humoral immunity.
Optim iza tion of Tissue Culture Conditions and Establishmentof Regenera tion System for Popu lus ×eu ram e ricana cl.‘J2’
ZHANG Lei, SU Xiao-hua, ZHANG Bing-yu, HUANG Qin-jun, ZHANG Xiang-hua, LI Yi-liang
2007, 20(6): 787-793.
Abstract:
The key factors affecting the growth of Populus ×euram ericana cl. ‘J2’in vitro and the op timal tissueculture conditions for it were ascertained. The high frequency regeneration system for P. ×euram ericana cl. ‘J2’was also established in this study. The op timum basic culture medium wasMS; the op timal subculture medium androot inducement culture medium for shootwere 1 /2MS + NAA 0. 08 mg·L-1 + IBA 0. 08·mg L-1 ; active carbon disadvantaged the growth of P. ×euram ericana cl. ‘J2’; photoperiod had little effect on P. ×eu ram ericana cl.‘J2’, while light intensity and the pH value of subculture medium had great effect on it, the op timal light intensitywas 2 500 lx and the op timal pH value was 6. 5; the op timal shoot inducement culture medium for leaf2exp lantwasMS + BA1. 0 mg·L-1 + NAA0. 1 mg·L-1 and for leafstalk-exp lantwasMS + BA 1. 0 mg·L-1 + NAA 0. 2 mg·L-1.
Growth Characters and The ir Correla tions with Fruit Y ield of ThirteenLarge Berry Cultivars of Sea Buckthorn in D ifferent Tr ia l Plots
ZHANG Jian-guo, DUAN Ai-guo, LUO Hong-mei, SHAN Jin-you, HE Cai-yun
2007, 20(6): 794-800.
Abstract:
Growth characters and their relationship with fruit yield of 13 Large Berry Cultivars of Sea buckthorn(Hippophae rham noides) were studied at two sites, Shuiling of Heilongjiang(47°13′N, 127°7′E) and Dengkou of InnerMongolia (40°20′N, 106°59′E). The results showed: ( 1) The length of Large Berry Cultivars has not obviousdifference between two trial p lots, the width of Large Berry Cultivars in Dengkou county was smaller than that inSuiling county, the difference of leafwidth and length varied with different cultivars; the leaf length of Chinese SeaBuckthorn was obviously smaller than that ofLarge Berry Cultivars, and the width was similar; the rate of leaf lengthto width in Dengkou was obviously higher than that in Suiling, the rate of leaf length and width can be viewed as anindex to compare the resistance and adap tation of different cultivars. (2) The number of branch thornswas differentamong different cultivars, and the amount was not equal for the same cultivar in different trial p lots, in Dengkou county, the amount of branch thorns had obvious increasing trend, which maybe an adap tation to drought, and thisadap tation was the reason why that Chinese Sea Buckthorn grew the most branch thorns. (3) The height, ground diameter and crown diameter of most Large Berry Cultivars in Dengkou were all lower than that in Suiling, thesegrowth indexes of Chinese Sea Buckthorn were obviously higher than that ofLarge Berry Cultivars. (4)Different cultivars could get to 10 000 kg?hm-2. (5) The correlation between grow and yield of differentLarge Berry Cultivarswas different, and yield could be effectively p redicted through growth indexes, but a perfect p rediction system stillneedsmore p ractical data to build.
Effects of Crown Attr ibutes onWood Character isticsand Increments of Popu lus ×xiaohe i
WANG Xiao-qing, LIU Xing-e, REN Hai-qing
2007, 20(6): 801-806.
Abstract:
Effects of crown attributes on wood characteristics of Populus xiaohei were studied . The results indicatedthat effects of crown attributes on basic density were not significant. ForMOE and comp ression strength parallel tograin, the effects were significant, while insignificant for MOR. Crown attributes exerted little impacts on fiberlength, fiberwidth and the ratio of fiber length to width. Effects of crown attributes on sapwood and heartwood areaat breast height were highly significant. MOE, MOR, and comp ression strength parallel to grain were found to benegatively correlated with both crown width and length. In addition, the linear regression model fitted the relationship between comp ression strength parallel to grain and crown width fairlywell, with R2 value of 0. 62. Fiber lengthand width, to a certain extent, were positively related to crown size. Sapwood and heartwood area were highly, positively related to crown size. Furthermore, a close linear relationship was found between sapwood area and crownwidth, with R2 value of 0. 78; while a second order polynomial equation were best in describing the relationship ofheartwood area with crown width, with R2 value of 0. 91.
The Character istics of Nutr ient D istr ibution in the Tree Crown ofAbies georgei var . sm ith ii Forest in Mount Sejila of Tibet
KONG Qing-yun, XIN Xue-bing, HUANG Jie
2007, 20(6): 807-813.
Abstract:
The forest in the Southeast Tibet is the key forest region and the ecology reserve in China. In virgin forestlocated on positioning station for research on ecosystem in Mount Sejila of Tibet, nutrients in the tree crown of fir(Abies georgei var. sm ith ii) forest were measured six times by samp le-p lot survey method and laboratorial analysisfrom May 13 to August 19, 2001. The objective was to research on nutrient contents in the leaves and branches according to different positions, directions or ages, and on amount of nutrient stored in tree crown of fir forest inMountSejila, in order to p rovide with the scientific foundation for forestmanagement in this region. Itwas showed that thetotal nutrient contents in annual leaveswere 1. 12 times that in perennial leaves, and that in the leaves exposed tothe sun were more than that in the sunless ones. The height of tree crown had influence on nutrient contents in leaves and there was some difference in nutrient contents among positions in the same branch. The characteristics of nutrient distribution in the branches were the same as in the leaves, therefore itwas showed that the branches and theleaves use nutrient simultaneously in the p rocess of its distribution or accumulation.
Genetic TraitVariation of Zenia insignis GeographicalProvenance in Seedling Stage
LIU Xin-hong, HE Xiao-yong, YUAN De-yi, WANG Jun-feng, GE Yong-jin
2007, 20(6): 814-819.
Abstract:
The seed ling experiments o f n ine Zenia insign is provenances from their ma jor distribution districts w ereconducted. The resu lts show ed that there ex isted extreme d ifferences in leaf w idth, leng th and area among provenances in seedling stage, w hich correlated w ith the seed size and thousand-seed w eigh.t There w ere not sign ificantdifference in seed ling heigh,t basa l diameter and biomass among provenances. Z. insignis adapts sunsh ine and w asheat-to lerance. The basa l diameter increm ent in July and August took 47. 38% ~ 51. 90% o f the annual to ta,l w hilethe he igh t increment took 62. 82% ~ 69. 06% of the annual tota.l So, the co ld-to lerance trait should be the key facto r in Z. insignis provenance selection. The individual se lection w as the key to cultivate fastgrow ing fine varieties.
So il Respira tion of the Representa tive Artif ic ia l Forests Dur ing theM inor Growing Sea son in the Hilly Reg ion of North-China
WANG He-song, ZHANG Jin-song, MENG Ping, GAO Jun, JIA Chang-rong
2007, 20(6): 820-825.
Abstract:
Soil resp iration of two rep resentative artificial forests during the minor growing season in the hilly region ofNorth2China, in case of 30 years old Platycladus orienta lis and 25 years old Ouercus variabili, were analyzed. Soilresp iration rate wasmeasured by L i-8100 automatic monitoring system, and the soil temperature and soilwater content wasmeasured by AR-A-ECH automatic monitoring system from Nov. of 2005 toMar. of 2006 . The main results are as follows: ( 1 ) For both the two artificial forests, soil resp iration rate ( SRR ) in clear-cloudy day wasmarkedly higher than that in overcast day, but the diurnal variation of SRR was not apparent under the two weatherconditions. Daily SRR changed significantly, and characterized with that SRR decreased sharp ly from November toJanuary of the next year, and maintained at a relatively low value in February, and then increased rap idly after themiddle ofMarch. Snowingmade SRR rise varying extents during this season. The average SRR of Platycladus orienta lis and Ouercus variabiliwere 0. 61μmol?m-2 ?s-1 and 0. 39μmol?m-2 ?s-1 respectively in the whole minor growing season. (2) Significant relationship were found between the two artificial forests SRR and soil temperaturesat the 0, 5, 10, 15 and 20 cm dep th from the soil surface ( p -2 ?s-1 and 1. 60-μmol?m-2 ?s-1 respectively. SRR was also correlatedwith the soilwater content ( p < 0. 01) , the regression equation for Platycladus orienta liswas linear, butmultinomialfor Ouercus variabili. SRR was significantly related to soil temperature ( Ts) and soil water content ( SW ) at 5 cmdep th. Ts was the most important factor that effected on SRR for both the two artificial forests through comparing thepartial coefficient of Ts and SW at the 5 cm dep th.
Research on Vegetation Structure and Evolvementof Limestone Mountain in Shandong Province
FANG Yong, DU Ning, WANG Shu-jun, WANG Yue-hai
2007, 20(6): 826-834.
Abstract:
The area suitab le for p lanting forests in Shandong Prov ince aremostly in limestonemounta in. Because ofits bad env ironmental cond ition, it is d ifficu lt to plant trees in limestonemounta in. W e did researches in 2001 and2005 respectively in order to apply theo ry to vegetation resume in limestonemoun tain. Based on community investiga tion, tw o representat ive limestone mounta in fo rests w ere se lected in Y iyuan and Boshan w hich w ere investigatedtw ice at 2001 and 2005. The land w as div ided into 4 types in both places, and 2~ 6 quadrats w ere placed in everykinds of area types. Then arbor layer ( 20 m × 20 m) , shrub layer ( 3 m × 3m ) and herbage layer ( 3 m × 3 m)w ere investigated separate ly. By means o f spec ies importance value, species diversity, n iche et al. , the eco log icalcharacter istics of vegetation in limestone mounta in were stud ied actually. Accord ing to comparison o f the data in2001 and 2005, w hich stand for the vegeta tion actuality under jamm ing and natural restorat ion actuality under forestenclosing and tending, the vegetation evo lvement w as stud ied of lim estonemounta in in Shandong Prov ince. The resu lts show ed that the actua lity o f limestone mountain in Shandong Prov ince w as comparat ive ly bad; the communitylevels w ere not dist inc.t The dom inant spec ies in shrub and herbage layers w ereVitex negundo andZ oysia jap onica,acco rd ing to fo rest enc losing and tend ing, the dom inant spec ies in shrub and herbage layers d id not change obv ious-ly. The vegeta tion in tw o spots w as in the process of evo lvem en,t but it d id not come to the ex tent o f succession.The cond ition w as better and the evolvement extentw as deeper in Y iyuan than in Boshan. The variety of species d iversity in shrub and herbage layerssw ere not identica,l and it rose of shrub layer in both spo ts. As for herbage layer,it dec lined in Boshan ( from 1. 54 to 1. 09) and rose in Y iyuan ( from 1. 22 to 1. 24). There w as no rule of speciesdiversity a long w ith alt itude. Re la tionships among impo rtance value ( IV ) , n iche breadth (BL) and n iche overlap(Op) in 2001 w ere not prom inen,t but itw as prom inent in 2005, it had the change trend from no linear corre lationto linear corre lation, from linear corre lation to logarithm ic correlation.
Effects of Several Phenolic Acids on Germ ination ofPhy llostachys heterocycla Seeds
ZHENG Ren-hong, GU Xiao-ping, YUE Jin-jun, SU Wen-hui
2007, 20(6): 835-840.
Abstract:
A fter cont inual covering for several years, the y ield and quality ofPhyllostachy s p raecox w ill decline seriously. A imedat at th is problem, w e detected the effects from a llelo-chem ica ls of the materia ls w hich are used forcovering. The so lution of several pheno lic ac ids, includ ing p-hydroxy benzo ic acid, van illic acid, p-coumaric acid,feru lic acid and the irm ix ture, and d istilled so lu tion o f different concentrat ions from rice straw and bamboo leavesw ere used on germ ination o fP hyllo stachys heterocy cla seeds. Mother d istilled so lution made radical dead in a shorttime, but bo th 5 and 10 t imes of mo ther solution promoted length and w e igh t of radica;l 100 mg?L- 1 is the low estconcentration o f pheno lic acids so lution and the irm ix ture, the leng th o f rad icalw hich treated by them was 24. 49%~ 48. 99% less than the contro,l 9. 17% ~ 38. 08% w as the w eigh;t compare w ith 100 mg? L- 1, the length andthe w eight o f radicalwh ich treated by 500mg?L- 1 w ere 73. 48% ~ 88. 63% and 76. 00% ~ 90. 83% less than thecontro l, respectively.
Studies on the Composition and D iversity of the CanopyArthropod Commun ity of Moso Bamboo Forest
LIU Huai, ZHAO Zhi-mo, XU Xue-qin, LI Ying-ping
2007, 20(6): 841-846.
Abstract:
The survey of canopy arthropod community was conducted by netting from Ap ril to October in 1999 inChangning Bamboo Area, Yibing, Sichuan. The results indicated that there were 113 species ( group s) of canopy insects, which belonged to 13 orders and 54 families method. All sp iders were considered as one group because ofsimilar ecological functions. Homp tera and sp iders constituted the dominant group s in arthropod communities in thefour investigation areas. The relative abundance ranged from 30% to 51% for Homp tera and from19% to 43% forsp iders, respectively. Dominant concentration index of Simp son was in the order ofAreaⅡ >AreaⅢ >AreaⅣ >AreaⅠ. Em poasca sp. (Homp tera: Cicadellidae) was the a dominant species in four Areas. At p resent, it had already become one of the most important bamboo pests in China. The indices of dominant concentration and diversitywere used to analyze and compare the seasonal dynamics of arthropod community. The dynamics of dominant concentration started highly, then decreased to a low level, but finally increased to a relatively high level. The changetendencies of the dynamics of diversity (H′) were from low to high, then to low, and to high again, and to low finally. This trend was closely related to bionomics of arthropod and seasonal change, but there was difference in the four areas. The dynamics of the diversity in Area II was an excep tion as a result of the obvious dominance ofEm poasca sp. inMay. Its change tendencywas from high to low, then to high, and to low again, and to high again,and to low finally. The seasonal pattern of bamboo canopy arthropod communitywithin investigation period could bedivided into five stages by the op timal sorting. The five stageswere before earlyAp ril, from mid Ap ril to early June,from mid June to early Sep tember, from mid Sep tember to mid October, and after mid October, respectively. Thesecond stage forming the first peak was the most important and op timum time to monitor dynamics and control pestsbecause most pests appeared at this stage. At the third stage, the pests declined because of high temperature andoccasional strong wind. At the forth stage, temperature declined to a relatively suitable range. The second peak ofsome insect pests appeared and sp ider population also increased. Biological control should be emphasized at thisstage. The arthropod over-wintered between late October and next earlyAp ril. Forestmanagement should be emphasized to control the over-wintering pest populations.
Physiolog ica l and Biochem ica l Changes of PoplarInfected by Ice Nuclea tion Active Bacter ia l Canker D isea se ( Ⅱ)
MAO De-jiang, ZHU Ya-ling, IANG Cun-ti
2007, 20(6): 847-853.
Abstract:
The ice nucleation activation bacterial canker disease decreased the content of mobile p roline in pop larbarks, while caused the content of bark pectin to increase a bit. Though the double2glucose content in infected barkwas less than that in healthy one, the glucose content in the infected bark wasmore above 25 ℃ and less below 20℃ than that in the controlmaterial. When the infection was not serious, itmade the content of chlorophyll in infected bark increase, yet the serious infection made the host had opposite appearance.
Study on the Factors Affecting the Regeneration ofin vitro Leaves of Sophora japonica f. 'L iao Hong'
QIU Yan-chang, DUAN Zu-an, ZHANG Jin-fang, ZHANG Xian-guang, LIU Wei
2007, 20(6): 854-858.
Abstract:
The propagationa l mu ltiple o f Sophora japonica .f ‘L iao Hong’ tube seedlings w as less affected by theconcentration o f sucrose, but the concentration o f sucrose affected the grow th morpho logy o f tube seed lings. Whenthe sucrose concentration increased from 20 g? L-1 to 60 g? L-1 inWPM m edia, the leaves turned to norma l fromcrink led and became dark g reen. The procedures of leave regenerat ion cu lture are as fo llow s: The d ifferentiatedseedlings o fS. jap onica w ere inocu lated onWPM ( w ith 6- BA 2. 0mg? L-1 + NAA 0. 05mg? L-1 + sucrose 20~60 g? L-1 + agar 70 g? L-1 ) , after cu ltured for 20 days, the d ifferent iated tube seed lings w ere obta ined. Theleaves sheared w ere put into asepsis w aterw ith 1mg? L-1 V itam in C and soaked for 1~ 3m inutes, then planted ontoWPM ( w ith 6- BA 2. 0mg? L-1 + NAA 0. 1 mg? L-1 + sucrose 40 g? L-1 + agar 7. 0 g? L-1 ) , and culturedunder illum ination for 30~ 40 days, the differentiated cutt ing tissues w ere go.t Cultured under contro lled env ironment ( illum ination tim e 12 hours, dark culture for 12 hours, light intensity 2 000 lx, temperature 25 , dark cu lture tempera ture 15 , pH 5. 8) for 20~ 30 days, the green callus were induced; then they w ere transplan ted onWPM med ia (w ith 6- BA 4. 0mg? L-1 + NAA 0. 05mg? L-1 + GA 2mg? L-1 + sucrose 60 g? L-1 + agar 7?0 g? L-1 ) , the adventitious budsw ere germ inated from green callus t issues, and the regenerated adventitious buds ra tew as 89% .
Fire RetardantMechanism of the Non-managed andPartlyManagedMulti-layer Firebreak Forests
HONG Chang-fu
2007, 20(6): 859-863.
Abstract:
Non-managed and partlym anaged fire retardantmulti-layer f irebreak forests w ith h igh fire retardant efficiency and low management costw ere estab lished in mountain gu llies and on low ridges using var ious afforestationmeasures, and fire retardantm echan ism w as analyzed by investigat ing re lated fire retardant factors. Results show edthat non-m anagedmulti-layer f irebreak forest belt got the h ighest fire retardant effic iencyw ith the h ighest capac ity oflum inous energy reduction, temperature decreasing, mo isture keeping and w ind break ing. Hum id ity ratio in liv ingplants layer, lither layer and so ilw as also the h ighest in investigated forest types. F ire retardant efficiency of thepartly managed mult i-layer firebreak forest be lt w as less than the non-managed be l.t And the sing le layer firebreakforest on uppermountain ridges show ed the least f ire retardant effic iency. Based on these resu lts, suggestsw ere g iven on non-managed and partlymanaged fire retardantmulti-ayer firebreak forest estab lishment bym aking use of themo ist env ironment w ith remained broadlea f forest inmounta in gu llies and low ridges near the gu lly. Suggestionsw erealso g iven on reconstruction o f the ex ist ing single layer firebreak forest on uppermountain ridges.
A New Species of Bashania Keng f. etY i onW estern Yunnan, China
SHI Jun-yi, YI Tong-pei, YAO Jun, YANG Lin, WANG Hai-tao
2007, 20(6): 864-866.
Abstract:
This paper reported a new species o f theBashania K eng .f e tY i from W estYunnan, Ch ina. New speciesis Bashania yongdeensis Y i et J. Y. Sh.i
Progress on the Code of ForestHarvesting Practice Based onthe Reduced Impact Logging
ZHANG Hui-ru
2007, 20(6): 867-871.
Abstract:
This paper introduced reg iona l and nat iona l codes of forest harvest ing pract ice in the wo rld based on thereduced impact logg ing, wh ich included FijiNat iona lCode of Logg ing Practice, FAOMode lCode of Fo restH arvest ing Practice, Code of Pract ice for ForestHarvesting in A sia-Pacific and Na tiona lCode o fPractice for ForestH arvest ing in Ch ina. These codes took eco log ica l priorities, cen tering on people and efficiency as basic principles, its purposew as to control over cutting of fo res,t reduced impact of forest harvest ing on forest and env ironm en,t improve efficiency o f forest harvesting and w ood ut ilization, balance economy, eco logy and soc iety, and to advance sustainab leforestmanagem ent through improv ing of forest harvest ing techniques. A im ing at so lv ing the problems in implemen t ofN ational Code o fPractice for ForestH arvest ing in Ch ina, som e suggestions w ere put forw ard, such as classify ing andconstitut ing codes of forest harvesting practice for various reg ions, enhancing research so as to prov ide theoret icaland techn ica l suppo r,t and setting up effect ive monitoring and superv ising system for forest harvesting.
Antim icrob ia l Activ ity of n-butanol Extractfrom Pa rthenoc issus tricuspida ta Leaves
LIU Hua-ling, MA Xin-rong, SUN Zhen-yuan
2007, 20(6): 872-875.
Abstract:
The n-butanol extract from Pa rthenocissus tricuspidata leaves were examined for antimicrobial activity against five microbes by using filter papermethod. The results showed that the extract restrained the growth of Escherichia coli, Agrobacterium tum efaciens, B acillus subitilis and B acillus pum ilus,while inhibited the growth of yeast at acertain extent. And the minimum inhibitory concentrations (M IC) were 0. 25, 0. 3, 0. 25, 0. 3, 1. 0 g?mL - 1 respectively. Moreover the n-butanol extract also could restrained some bacterium strains, which had antibiotic resistances. After heated at 95 ℃ for 2~3 h, the n-butanol extract from P. tricuspidata leaves still p resented strong bacteriostasis to germs. This result indicated that the substance had heat-stability.
A New Record Genus(Leucogyrophana, Coniophoraceae, Basidiomycetes)and A New Record Species (L . pseudomollusa) in China
ZHOU Xu-shen, JIANG Jun-qing, LIU Gui-qin, DAI Yu-cheng
2007, 20(6): 876-878.
Abstract:
The genus Leucogyrophana Pouz. w as found from Yunnan Prov ince, sou thw est Ch ina, and itw as the firstreport from Ch ina. A cco rd ing ly, Leuogyrophana p seudomollusa ( Parm. ) Parm. w as the f irst reco rd o f the Chinesefungal f lora. L. p seudomollusa grew on rotten w ood ofP inus. The present paper provided a deta iled descr iption andillustration based on the Ch inese materia.l
Germ ination Test of Wild Ornamen ta l Spec ies L espedeza f loribunda
ZHENG Jian, LI Xin-feng, GUAN Nan, WU Chao, ZHENG Yong-qi
2007, 20(6): 879-882.
Abstract:
Germination of Lespedeza f loribunda Bunge. seedswas studied in order to p rovide cultivation and app lication in gardening and landscap ing. Results indicated that the seeds of Lespedeza f loribunda had the characteristic offorcing dormancy thatwas caused by hard seed vessels. The dormancy could be released by soaking in hotwater andby sulphuric acid treatment. The most app rop riate treatment time was 10~30 min, resulting in average germinationrate (96% ) , average germination power (82. 53% ) and average germination index (17. 54). The op timum germination temperature was 25 ℃ and the germination rate, germination power and germination index was 95% , 81% ,22. 95 respectively after treated by sulphuric acid for 20 min.
Stock Sap Flow Characters of Juglans reg ia and Relationship withM eteorological Factors in Dry Season
MA Chang-ming, ZHAI Ming-pu
2007, 20(6): 883-886.
Abstract:
Juglans reg ia is an important econom ic tree species. Observation of its stock sap flow w as carried bySF100 heat pulse recorder in dry season( April-July) in area of conversion farm land for forest and surround ing meteorolog ical facto rs such as air temperature, a ir relative hum idity and radiation intensity w ere monitored in-phase. Experimental p lot locates in P ing shan, H ebe.i Th is study w ould o ffer some references for vegetation restoration. Theresu lts show ed that the Jug lans reg ia stock sap flow changed obv iously betw een day and n igh,t w hich follow ed a single peak type curve. Variation amplitude w as b ig in sunny day and little in cloudy day; In Apr il-M ay, sap flow w asbig and little in June-Ju ly because of so ilw ater stress; In fluencing order on stock sap flow flux byme teoro log ica l facto rs w as sun rad iation> a ir temperature> re lative hum id ity respectively.