44 isolates of fungi were isolated from the xylem of the Pinus thunbergii samples(healthy stem,weak stem and withered stem)infected by the pine wilt disease caused by pine wood nematode,Bursaphelenchus xylophilus,from infected trees at Zhoushan and Pinghu of Zhejiang Province in China,and these isolates were identified as:Trichoderma spp.,Penicillium spp.,Pestalotia spp.These identified isolates took 70.62% of total isolates of the fungi.Among the 12 isolates unidentified,one sporiferous fungus was found,the other 11 isolates were nonsporiferous.Trichoderma spp.was the dominant fungus in P.thunbergii,the total rate was 53.34%,followed by Penicillium spp.,18.89%.
The partial correlation analysis on the relationship between the main meteorological factors and daily emergence amount of Carpomyia vesuviana overwinter generation adult from the beginning to the peak of emergence was conducted. The results of stepwise regression analysis show that the air temperature, the soil temperature and the relative humidity of soil are the main meteorological factors affecting the emergence amount of overwintering emergence. The results of Path analysis and decision-making system analysis show that the air temperature has positive affect on the emergence and is an important index directly impacting on the emergence amount of C. vesuviana; The soil temperature is the most important factor limiting the amount of emergence; the soil relative humidity has the greatest comprehensive influence on the amount of emergence. Based on partial correlation analysis, a multiple regression equation was established on the main meteorological factors and C. vesuviana overwintering generation adult emergence. The emergence amount predicted by the model is significantly correlated with the amount of emergence which was field measured. So this model is applicable for forecasting the amount of C. vesuviana overwintering generation adult.