[Objective] Using ArrayTube, a high-throughput microarray technology, to detect poplar canker pathogens in Beijing area. [Method] The DNA extracted from symptomatic and asymptomatic poplar bark were used to hybridizing by ArrayTube. The results of hybridization were recorded and analyzed by IconoClust-AT 3.0. Then the hybridization results were verified by cloning of the corresponding tissue. [Result] The results showed that Botryosphaeria dothidea and Valsa sordida were successfully detected as the target pathogens in symptomatic samples by microarray hybridization. The B. dothidea was also found as an endophyte in healthy tissue. [Conclusion] The results indicated that the ArrayTube technique is an effective method for quick detection of poplar canker pathogens, it could be further applied in other forest plant pathogen detection or diagnosis.