[1] Ryan C A. Systemic response to wounding[A]. In: Denno R, McClure M. Variable Plants and Herbivores in Natural and ManagedSystems[M]. Academic Press, New York, 1983: 43 ～ 60
[2] Ryan C A. Proteinase inhibitor gene families: Strategies for transformation to imp rove p lant defenses against herbivores [J]. BioEssays, 1989, 10: 20 ～ 24
[3] Ryan C A. Protease inhibitors in p lants: genes for imp roving defenses against insects and pathogens [J]. Annu Rev Phytopathol,1990, 28: 425 ～ 449
[4] Lipke H, Fraenkel G S, Liener I. Effect of soybean inhibitors ongrowth of Tribolium confusum [J]. Agric Food Chem, 1954, 2: 410～ 414
[5] Peng L H, Black L L. Increased p roteinase inhibitor activity in response to infection of resistant tomato p lants by Phytophthora infestans[J]. Phytopathol, 1976, 66: 958 ～ 963
[6] Geoffroy P, LegrandM, FritigB. Isolation and characterization of ap roteinaceous inhibitor of microbial p roteinases induced during thehypersensitive reaction of tobacco to tobacco mosaic virus [J]. MolPlant2Microbe In, 1990, 3: 327 ～ 333
[7] Pautot V, Holzer FM, Walling L L. Differential exp ression of tomato p roteinase inhibitor Ⅰand Ⅱ genes during bacterial pathogeninvasion and wounding [J]. Mol Plant-Microbe Interact, 1991, 4:284 ～ 292
[8] Roby D, Toppan A, Esquerre2Tugaye M T. Cell surface in p lantmicroorganism interactions. Ⅷ. Increased p roteinase inhibitor activity inmelon p lants in response to infection by Colletotrichum lagenarium or to treatment with an elicitor fraction from this fungus [J].PhysioMol Plant Pathol, 1987, 30: 453 ～ 460
[9] Hilder V A, Gatehouse A M R, Sheerman S E, et al. A novelmechanism of insect resistance engineered into tobacco [J]. Nature, 1987, 330: 160 ～ 163
[10] Bradshaw H D J r, Hollick J B, Parsons T J, et al. Systemicallywound2responsive genes in pop lar trees encode p roteins similar tosweet potato sporamins and legume Kunitz tryp sin inhibitors [J].PlantMol Biol, 1989, 14: 51 ～ 59
[11] Parsons T J, Bradshaw H D J r, Gordon M P. Systemic accumulation of specific mRNAs in respone to wounding in pop lar trees [J].Proc Natl Acad Sci, USA, 1989, 86: 7895 ～ 7899
[12] Hollick J B, Gordan M P. A pop lar tree p roteinase inhibitor-likegene p romoter is responsive to wounding in transgenic tobacco [J].PlantMol Biol, 1993, 22: 561 ～ 572
[13] HarutaM, Major I T, ChristopherM E, et al. A Kunitz tryp sin inhibitor gene family from trembling aspen (Populus trem uloidesMichx.) : cloning, functional exp ression, and induction by wounding and herbivory [J]. PlantMol Biol, 2001, 46: 347 ～ 359
[14] Nishiguchi M. A genomic DNA clone encoding tryp sin inhibitorhomolog [D].
[15] 秦锡祥, 高瑞桐, 曹桂洪, 等. 抗光肩天牛杨树品种的筛选研究[J]. 林业科学研究, 1993, 6 (专刊) : 42 ～ 45
[16] Larson P R, Isebrands J G. The p lastochron index as app lied to developmental studies of cottonwood [J]. Can J Forest Res, 1971,1: 1 ～ 11
[17] MurrayM G, Thomp son W F. Rap id isolation of high molecularweight p lant DNA [J]. Nucl Acids Res, 1980, 8: 4321 ～ 4325
[18] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A LaboratoryManual [M]. Cold Sp ring Harbor Press, 1989
[19] Towbin H. Electrophoretic transfer of p rotein from polyacrylamidegels to nitrocellulose sheets: p rocedure and some app lication [J].Proc Natl Acad Sci, USA, 1979, 76: 4350 ～ 4354
[20] 方敏,黄华樑. 包涵体蛋白质体外复性的研究进展[J]. 生物工程学报, 2001, 17: 608 ～ 612
[21] Bradford M. A rap id and sensitive method for the quantitation ofmicrogram quantities of p rotein utilizing the p rincip les of p rotein-dyebinding [J]. Anal. Biochem, 1976, 71: 248 ～ 254
[22] Kim S H, Hara S, Hase S, et al. Comparative study on amino acidsequences of Kunitz-type soybean tryp sin inhibitors, Tia, Tib, andTic1 [J]. J Biochem, 1985, 98: 435 ～ 448
[23] Richardon M. Seed storage p roteins: the enzyme inhibitors [J].Meth Plant Biochem, 1991, 5: 259 ～ 305
[24] 赵天锡,陈章水. 中国杨树集约栽培[M]. 北京:中国科学技术出版社, 1994: 269 ～ 307
[25] Yeh KW, Chen J C, Lin M I, et al. Functional activity of sporamin from sweet potato (Ipom oea batatas Lam.) : a tuber storagep rotein with tryp sin inhibitor activity [J]. PlantMol Biol, 1997,33: 565 ～ 570
[26] ConfalonieriM, Allegro G, Balestrazzi A, et al. Regeneration ofPopulus nigra transgenic p lants exp ression a Kunitz p roteinase inhibitor (KTi3) gene [J]. Mol Breed, 1998, 4: 137～145