Abstract: The present paper reports the processing techiniques of the formulation of nuclear polyhedrosis virus of Apocheima cinerarius, and its detection of safety.New formulation of the AciNPV were made by incorporating the preparation of polyhedrals inclusion bodies (PIB) with sunlight protectant and other abjuvants in a blend screen of electroshak.Bioassays tests indicated that samples of the aceton-precipitation and new formulations of virus were very effective for early third-instar larvae.The LC50 and 95% fiducial limits were 2.84×103PIB/ml, 2.84×103PIB/ml, and 1.70×103-4.76×103 PIB/ml and 1.43×103-5.66×103PIB/ml respectively.Several powder formulations were directly diluted with water and mineral oil (NO.0 diesel oil) when used in the field.The tests conducted on small plots and large blocks showed that several formulations of AciNPV were effective for the poplar looper 2-3 instar larvae in forest field.The mortality of poplar looper larvae was over 85% in 12-15 days.Safety tests showed that formulation of AciNPV was not hamper to domestic animals and did not couse mutation.It was safety for man.Bacteriological detection proved that pathogenic bacteria, such as Escherichia coli, Salmonell, Vibric, and Clostridium were cultured without isolation.