Abstract: The success of the artificial inoculation of pines with the vegetative inoculum of Pisolithus tinctorius (pt) is confirmed by means of making a comparison of cultures between reisolates from ectomycorrhizae formed and mother isolate inoculated, and an identification of sporocarps connected with the ectomycorrhizae;the gross form and anatomy structure of the ectomycorrhizae formed after inoculation are described by using the photos from the experiment.It is discovered that the typical ectomycorrhizae fungus, pt can form ectendomycorrhizae with pines under the condition of artificial inoculation in nursery.The application experiments conducted in nurseries give evidence that inoculum made by the Center for Mycorrhizal Research and Development,CAF is effective in increasing ectomycorrhizae,promoting the growth and improving the biomass of the seedlings,100% seedlings of four pines receiving vegetative Ptinoculum have got pt ectomycorrhizae with pt index 88+,the yield of plantable seedlings increased by more than 14.6%,and height,root-collar diameter, biomass, the number and the total length of the lateral root for the ten-month-old seedlings increased,on the average,by 28.1%～71.4%, 22.8%～49.2%, 149.2%～457.1%,150.0%～200.0% and 82.4%～101.1% respectively.