Abstract: It is well known that mycorrhizal associations are an integral component of natural and managed ecological system and most vascular plants to form one of the two most common associations:either ECM or VAM. However, in contrast, the genus Ecualyptus is capable of forming both ECM and VAM, even in the same root system. This paper represents the analysis results on physiology of ecto and endomycorrhizas of E. urophylla colonised by both Glomus caledonium and Pisolithus tinctorius isolates in nursery. The root activity and the concentration of total amino acids in root tissue fluid of coinoculated seedlings increased by 28.69%～31.90% and 5.20～5.22 times respectively compared to that of uninoculated ones 5 months after inoculation. Polysaccharides concentration in mycorrhizal roots varied according to different fungal treatments, and were generally 2.11～2.75 times of that of the controls. However, that in roots of seedlings which colonised by both ECM and VAM fungi was extremely lower when comparing to that inoculated with individual fungus. The changes of physiology reflect inoculating effect on plant growth.