Abstract: On the test of tissue culture,using shoot tips of Actinidia chinensis as explant,the highest rate of shoot differentiation is 79.1%,and the shoot differentiation rate of lateral shoot and stem are respectively 45.8% and 24.4%,and the shoot differentiation rate of blade is 0%. On the test of shoot differentiation and subculture,using shoot tips as explant,and the improved MS culture medium by increasing 1.5 mg/L BAP and 0.2 mg/L NAA,and half decreasing N element,the rate of shoot differentiation can reach 97.7%. It can obviously improve the coefficient β1 of subculture to add 10 mg/L glutamine on the test of subculture,when it comes to the third time of subculture, β1 can get to 127.5,however the check β1 is only 12.7. Taking MS culture medium on half decreasing N element or N element and Fe salt as taking root culture medium,the rooting rate can get to 100%,which increases 30% more than the comparison. The average root length are respectively 4.2 cm and 7.1 cm longer and the rooting time are respectively 3 days and 6 days earlier than those of the comparison. Adding 3% active carbon to rooting culture medium can further increase the root length and shorten the rooting time. The research holds that reproductive coefficient ε is the main index to measure how many planting stock one explant can reproduce. It is ε =101 on this test,which means that one explant can reproduce 101 planting stock.