Abstract: species of bamboo in 20 genera were cultured in vitro in recent 20 years.Plant regeneration via somatic embryogenesis and adventitious shoot was achieved in callus cultures derived from explants excised axially buds,shoot apices or mature zygotic embryos.Liquid suspension-culture cells of bamboo served as protoplasts donors,and high yields of viable protoplasts were obtained,and callus from protoplasts of suspension cultured bamboo cells were recovered. About 70 species of bamboo tested were successfully propagated in vitro . For nearly each species,multiple shoots were produced from axially buds on stem node segments cultured on MS medium containing BA. A few callus plant could be regenerated adventitiously from callus. In vitro flowering was induced on shoot developed from nodal explant taken from regenerated plants. The flowering of bamboo shoots of some species occurred in unison,but not that of other species.