Abstract: Experimental results demonstrated various responses of two geographical strains of Tricholoma matsutake (9924 and 99606) in the culture conditions.The suitable temperature ranges for the growth of strains 9924 and 99606 were 10～25 ℃ and 15～25 ℃ respectively.The suitable pH ranged from 3.5 to 7.5 for strain 9924 while relative short range (4.5～5.5) for strain 99606.Regarding the requirement of carbon,polymeric glucose was the optimum sources for strain 9924,and glucose,frustose,maltose and starch were also suitable for its growth.Comparably,cornpowder was the best carbon source for the strain 99606 and followed with glucose,dextrin,maltose,sucrose,starch,etc..Both strains were able to absorb such nitrogen sources as (NH4)2SO4,NH4Cl,NH4NO3 other than (NH4)2HPO4 or urea.Results also showed that vitamin could benefit the growth of both strains,especially for the strain 99606.