Abstract: Two Tricholoma matsutake strains,9924 and 99606 initially collected from Yunnan,China and Japan respectively,were utilized to inoculate seedlings of 6 pine species,namely Pinus yunnanensis,P.armandii,P.massoniana,P.elliottii,P.caribaea and P.taeda.Results indicated that about 75% samplings of P.yunnanensis and 78.1% of P.elliottii were infected at 90 days after inoculation.After 180 days,all inoculated saplings of the 6 pine species formed mycorrhizal association with average infective rate of 90.63%～100%,and 50 00～84 68 for Mycorrhizal Index. Morphological characteristics of mycorrhizae varied with different plant species,and they were normally occur unbranched,dichotomously branched and densely cluster like.The total mycorrhizal length also was diversified as longest in P.massoniana root system while shortest in P.armandii.Slight differences on the mantle density and the Hartig net usually found on each plant strain combination were illustrated.