Abstract: Several activation agents were used to treat various phosphate fertilizers and their biological effect and mechanism in soil under the eucalypt plantation on basalt latolol were studied. The results are as follows: (1) The growth of eucalypt increased markedly in soil under the eucalypt plantation on basalt latolol when potted plants were tested by a few activation matters to examine the phosphatic manure. The phosphorus powder treated by activation agent No. 2 can promote the increment of eucalypt trees significantly. The feasible concentration of organic activation agent is 5%. (2) The Fe and Mn elements in the plantation soil are closely correlated to the efficiency of P element, and the soil Mn has greater fixation effect on P under the condition of reduction. Si can benefit the release of P in soil, and proper Ca content can also increase the activity of P element. (3) The activation agents tested can not only promote the release of hard soluble P, but also control the release of water soluble P so as to stabilize the P supply from soil. (4) In the basalt latolol soil eucalypt system, the promotion and control mechanism of activation agents to P is to adjust the contents of Fe, Mn and Al in soil so as to realize the activation of P.