Abstract: Systematic research was taken on in vitro rapid propagation of the hybrid of 28 year old (Bambusa pervariabilis×Dendrocalamus latiflorus)No.7 The results are as follows:(1) Both reducing the salt concentration of MS medium and raising BA(6-Benzyl aminopurine) consistency favor the forming of clumping shoots; (2) In 9 sets of mediums for shoot proliferation, which were designed according to the diagonal intersection, the difference of shoot increase rate is not notable, the growth capacity of shoots differs near 4 times and in all the formula, if with the same BA concentration, the growth condition of the plantlets is better with KT(Kinetin) than that without KT in medium, which indicates that KT has an improving function on shoot growth; (3) The medium of 3/4 MS+BA 2mg·L-1+KT 1.25mg·L-1is fit to shoot proliferation; (4) New shoots are easy to root, culturing shoots in rooting medium for 20～30 days and then in proliferating medium for 5～7 days can make new shoots come up synchronously, the new shoots are cultured in rooting medium again and the rooting rate can achieve 78.2%. In this way the problem of shoot dying in rooting medium is solved. Following the experience mentioned above, many species such as Dendrocalmus minor var. amoen and Dendroclamus asper ect.(all are more than 30 years old) have been succefully cultured.