Abstract: The map was constructed using Populus tomentosa × P.alba var. pyramidalis × P. tomentosa hybrid population in which the resistance of Dothrorella gregaria Sacc was segregated.The authors had obtained 662 markers using 19 PstI/Mse1 primer pairs and 4 EcoRI/Mse1 primer pairs,of which 43. 2% had 3∶1 in segregation,14. 5% appears to be non-parental heteroduplex,and 48. 8% was distorted.239 markers had been mapped into 22 linkage groups (each including 4 loci or more),and the integrated map was 4 418 cM in length,with an average locus interval of 18. 5 cM.Linkage group LG1 was the largest one and covered 524 cM with 25 markers.The densest linkage group was LG13 with an average interval of 6. 7 cM in length.There were duplicated loci for 8 markers,and 7 markers that linked to one of markers in a linkage group but couldn′t be mapped into that group.In addition,there were 15 minor groups harboring 2～3 markers belonged to the parents.Moreover,13 and 21 groups belonged to paternal and maternal parent,respectively.