Abstract: It was demonstrated by cutting experiment of Elacocarpus sylvestris,Liquidambar formosana,Altingia gracilipes,Magnoliaceae skinneriana,Temstroemia gymnanthera,with semi-lignified branches treated by different root-inducing regulator and concentration and substrate at Changle Forest Experamental Station of Yuhang District of Zhejiang in June 2003 year that cutting survival rate,rooting rate,rooting coefficient,length of the roots and the quantity of the new roots of 5 tree species had significant different after six month,and it was different that the reaction of different tree species to rooting-inducing regulator.The best treatment combination of 5 tree species was:Elacocarpus sylvestris:NAA, 250 mg·L-1,rooting rate 97.0%;Liquidambar formosana:IBA,250 mg·L-1,rooting rate 64.5%;Altingia gracilipes:ABT1, 250 mg·L-1, rooting rate 74.6%;Magnoliaceae skinneriana:IBA,500 mg·L-1,rooting rate 56.1%;Temstroemia gymnanthera:IBA,250 mg·L-1,rooting rate 95.2%.The different cutting substrates had significant influence on rooting rate.The substrate No.2 (yellow soil:river sand =7∶3(volume ratio)) was best.