Abstract: The communities'structure, density, biomass and diversity of soil macrofauna in five habitats, which are zonal forest (original forest), secondary forest, brush, grassland, and Pinus yunnanensis forest, representing the secondary-succession karst vegetation bushes and grass land with P. yunnanensis, and making up a series of local forest succession from human interference, had been studied respectively in rain season and dry season in the world-famous Shilin karst scenery park. The result from the study showed that the groups, biomass, diversity indexes of soil macrofauna there clearly depended on human interfering situation and history. The dominant groups of soil macrofauna are the Castopoda, Diplopoda, Hymenoptera, Lumbricida, and Lumbricida was only found rich in original forest and secondary forest. Therefore, Lumbricida is an important species for indicating ecological environment. No matter it is rain season or dry one, the number of groups of soil macrofauna, density, biomass and diversity index were the greatest in the original forest, and lowest in bushes and grassland which were most heavily disturbed. There was a clear phenomenon which soil macrofauna were accumulated in surface soils. The coefficient of communities of soil macrofauna found in 5 habitats was low, but it was the most obvious in the secondary forest, which was protected and naturally restored, and the zonal forest remaining because of local traditional religion.