Abstract: In this paper, 6 provenances including 8 plus trees of 30-month-old Eucalyptus wetarensis were selected in an orchard in Jiangmen City, Guangdong Province for controlled pollination. Many hybrids were acquired by breeding with different pollen of Eucalyptus trees. The results indicated that:(1) average value of fruits/sack among different pollens is in the order of E. urophylla'5 E. wetarensis' E. grandis', the average seed quantities per fruit is in the order of E. urophylla 's E. wetarensis ' E. grandis':(2) incision methods of flower pillar for controlled pollination could acquire hybrids and save pollination time;(3) all diallel crossing could in favor of analysis of genetics between parents and progenies, but isolation of phonological phase would prolong period of breeding to attain object hybrids ; (4) the average value of fruits/sack was significantly different among different mother trees according to variance analysis, and the order of value is W3102 's W3507 's W3604's W3401 's W3708 's W3206 's W3103 's W3105 's. The impact of mothers for value of fruits/sack was much higher than pollens', so it is very important to select mother trees in breeding.