机械损伤对复叶槭叶片过氧化氢含量的影响
Effect of Mechanical Wounding on the Content of HydrogenPeroxide in Ash leaf Maple Seedlings
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摘要: 以复叶槭为实验材料, 采用DAB组织染色和分光光度法, 定性定量地检测了人为机械损伤和外源茉莉酸甲酯处理0、15、30、60、120、240 m in后损伤叶片, 系统叶片以及诱导叶片内H2O2含量的变化情况。结果显示, 机械损伤可诱导H2O2系统性产生, 无论是受伤叶片、系统叶片还是诱导叶片, 在伤害处理60 m in 内, H2O2含量一直增加, 240 m in后含量达最大值后开始下降。用1 ??mo l·L-1茉莉酸甲酯熏蒸健康植株后, 叶片H2O2含量显著增加。证明茉莉酸甲酯作为植株体内伤信号的报警信号分子, 能代替机械损伤等外界刺激启动相似的信号防御系统, 起到防御外界伤害的作用。Abstract: Using ashleafmaple (Acer negundo ) seed lings as materials, DAB tissue coloration and spectropho tometryasm ethods, the autho rs exam ined and compared the dynam ic concentrations o fH2O2 in d ifferent leaves: the dam??aged leaves, the intact leaves of the ident ical damaged plant and signa l induced leaves o f the neighboring plantsdamaged by 0, 15, 30, 60, 120, 240m inutes. The resu lts show ed that themechanicalwound ing cou ld induce the sys??tem ic concentrations of H2O2, the contents susta ining increased in 60m inutes, and reached the m ax po int at 240m inutes. A fter using theM eJA ( 1 ??mo l·L-1 ) to treat the hea lthy p lants, the concen trat ions o fH2O2 increased dis??t inctly. It could be concluded thatMeJA induced the sim ilar defensive system s instead o fmechan ica lw ounding, byw ay of initial signa ls in plants, and reached the effects of defending env ironmen tw ound.
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