Abstract: The tender leafstalks of Ledum palustre var. dilatatum were used as explants for the experiment. Uniform design for the most suitable media for shoots regeneration immediately at base of tender leafstalks, rooting and germplasm conservation in vitro was screened. The results showed that N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1 was fits for shoots regeneration, the frequency of shoots induction was higher than 92.5%; MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1 for rooting, the rate of rooting was 98%; N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1 for germplasm conservation in vitro for 46 months. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 90-fold proliferation rate was achieved within 30 days. The method of "deferring growth with dwarfing" was utilized for germplasm conservation in vitro at normal temperature. In vitro culture and germplasm conservation in vitro system of Ledum palustre var. dilatatum was established.