不同基因型欧洲黑杨幼苗氮素利用效率差异及其机理初探
Study on Difference and Primarily Mechanism of Nitrogen Use Efficiency (NUE) in Populus nigra Seedlings Genotypes
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摘要: 以104份欧洲黑杨不同基因型(无性系)为材料,通过生长量和生理指标的测定,研究土壤施氮和不施氮条件下其氮素利用效率的差异。结果表明:不同供氮水平下,不同基因型欧洲黑杨生长差异显著。根据2个供氮水平的苗木年平均材积生长量将欧洲黑杨群体划分为4个类型:双高效型、高氮高效型、低氮高效型和双低效型,并结合氮反应指数在双高效型与高氮高效型中分别筛选出8个氮素利用效率高、生长表现优良的基因型,具较高的育种价值。比较分析欧洲黑杨不同基因型氮素利用效率相关指标,发现双高效型的净光合速率、硝酸还原酶和谷氨酰胺合成酶活性、根系干质量、体积、比表面积均高于高氮高效型,初步揭示了不同类型欧洲黑杨氮利用效率存在差异的机理。Abstract: The variation in nitrogen utilization efficiency of 104 Populus nigra genotypes was studied by analyzing growth and physiological parameters under nitrogen fertilization or non-fertilization conditions. The results showed that the variation of nitrogen utilization efficiency among seedlings of varied Populus nigra genotypes were significant at different nitrogen supply. All seedlings with varied genotypes in this study were divided into four categories according to annual average individual volume increment in the two nitrogen treatments. These categories are high efficient in both nitrogen applications, efficient in high N application, efficient in low N application, and low efficient in both nitrogen applications. Eight genotypes were selected from each of the first two categories. These seedlings are high efficient to nitrogen utilization with excellent growth and productivities, therefore they are highly valuable for tree breeding. Comparison and analysis of the related parameters of nitrogen utilization efficiency showed that the the values of nitrogen response parameters including net photosynthetic rate, nitrate reductase activity, GS Activity, root dry weight, root volume and specific root area of seedlings within the first category were all higher than those within the second category, which reveal preliminarily the mechanism underlying nitrogen utilization of different Populus nigra genotypes.
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[1] 方升佐. 中国杨树人工林培育技术研究进展[J]. 应用生态学报, 2008,19(10):2308-2316 [2] 肖 君, 方升佐. 杨树人工林施肥效应研究进展[J]. 林业科技开发, 2007,21(4):15-17 [3] 曹桂兰, 张媛媛, 朴钟泽,等. 水稻不同基因型耐低氮能力差异评价[J]. 植物遗传资源学报, 2006,7(3): 316-320 [4] 刘建安, 米国华, 张福锁. 不同基因型玉米氮效率差异的比较研究[J]. 农业生物技术学报, 1999,7(3): 248-254 [5] Karim S A, Hawkins B J. Variation in response to nutrition in a three-generation pedigree of Populus[J]. Canadian Journal of Forest Research, 1999,29(11): 1743-1750 [6] 张 曦, 胡增辉, 苏晓华, 等. 土壤氮水平对几种黑杨新无性系苗木生长的影响[J]. 现代农业科学, 2009,16(1): 80-82 [7] 马祥庆, 刘爱琴, 黄宝龙, 等. 氮素高效基因型杉木无性系的选择研究[J]. 林业科学, 2002,38(6): 53-57 [8] 樊瑞怀, 杨水平, 周志春, 等. 氮素营养对马褂木家系苗木生长效应分析[J]. 林业科学研究, 2009,22(1): 85-90 [9] 丁明明, 苏晓华, 黄秦军. 欧洲黑杨基因资源稳定碳同位素组成特征[J]. 林业科学研究, 2006,19(3): 272-276 [10] 张香华, 苏晓华, 黄秦军, 等. 欧洲黑杨育种基因资源ssr多态性比较研究[J]. 林业科学研究, 2006,19(4): 477-483 [11] 刘文环, 李朝晖, 姜海涛, 等. 杨树扦插苗追肥试验[J]. 防护林科技, 2000(3):70-71 [12] 高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006: 61-64 [13] 唐启义, 冯明光. 实用统计分析及其DPS数据处理系统 [M]. 北京: 科学出版社, 2002: 49-265 [14] 余树全, 付达荣, 李翠环, 等. 康定杨优树无性系苗期测定[J]. 浙江林学院学报, 2003,20(3): 245-248 [15] 张亚丽, 樊剑波, 段英华, 等. 不同基因型水稻氮利用效率的差异及评价[J]. 土壤学报, 2008,45(2): 267-273 [16] 宋金耀, 刘永军, 党晓燕, 等. 新山海关杨速生性的主因子分析[J]. 中国农学通报, 2008,24(7): 384-389 [17] 余树全, 付达荣. 高原杨树叶片硝酸还原酶活性对比差异初步研究[J]. 四川林业科技, 1997,18(1): 51-54 [18] 周国璋, 王世绩. 四种杨树扦插苗的硝酸还原酶活力体内衰减的初步研究[J]. 林业科学研究, 1989,2(3): 296-299 [19] 曾建敏, 崔克辉, 黄见良, 等. 水稻生理生化特性对氮肥的反应及与氮利用效率的关系[J]. 作物学报, 2007,33(7): 1168-1176 [20] Bertrand H, Pascal B, Isabelle Q, et al. Towards a Better Understanding of the Genetic and Physiological Basis for Nitrogen Use Efficiency in Maize[J]. Plant Physiology, 2001,125: 1258–1270 [21] Bahman E, John R S, Jerry W M. Fractal Analysis for Morphological Description of Corn Roots under Nitrogen Stress[J]. Agronomy Journal, 1993,85: 287-289 [22] 程建峰, 潘晓云, 刘宜柏. 土壤条件对陆稻根系生长的影响[J]. 土壤学报, 2002,39(4): 590-598
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