运用AFLP技术分析筇竹种群遗传多样性
Analysis of Populations Genetic Diversity of Qiongzhuea tumidinoda Using AFLP Markers
-
摘要: 利用AFLP分子标记技术对筇竹2个种群的遗传多样性进行了分析,筛选出10对多态性和清晰度较高的引物组合,共获得680个AFLP位点,其中多态性位点662个,平均多态性检出率为97.20%,并用PopGen32软件对AFLP多态性数据进行分析,结果表明,供试2个筇竹种群均具有丰富的遗传多样性,多态位点百分率分别为89.86%和91.95%,等位基因数分别为1.898 6和1.919 5,有效等位基因数分别为1.585 0和1.568 3,种群内遗传多样性为0.332 1,种群水平平均Nei’s 遗传多样性为0.394 9,Shannon信息指数为0.575 4,基因流为2.900 9,遗传一致度为0.821 9,遗传距离平均值为0.196 1。并根据遗传距离进行了UPGMA聚类分析。Abstract: Amplified fragment length polymorphism (AFLP)was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers, Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic(97.20%). PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%; the number of alleles were 1.898 6 and 1.919 5; the effective number of alleles were 1.585 0 and 1.568 3; the average genetic identities within 6 populations was 0.332 1; the average genetic identities within the 2 populations was 0.394 9; Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.
-
Key words:
- Qiongzhuea tumidnoda
- / genetic diversity
- / AFLP
-
[1] 傅立国,金鉴明.中国植物红皮书——稀有濒危植物:第l册[M].北京:科学出版社,1992:318-319 [2] 袁金玲,熊登高,胡炳堂,等.珍稀保护竹种筇竹笋营养成分的研究[J].林业科学研究,2008,21(6):773-777 [3] 董文渊,黄宝龙,谢泽轩,等.筇竹秆花结实特性的研究[J].南京林业大学学报,2001,25 (6):30-32 [4] 袁金玲,熊登高,金 光,等.珍稀保护竹种筇竹引种育苗研究[J].林业科学研究,2009,22 (2):166-170 [5] 周延清.DNA分子标记技术在植物研究中的应用[M].北京:化学工业出版社,2005 [6] Loh Jin Phang,Ruth Kiew,Ohm Set,et al..A study of gentic variaton and relationships within the bamboo subtribe Bambnsirlae using amplified fragment length polymorphism [J]. Annals of Botany,2000,85:607-612 [7] Marulanda M L,Marquez P, Londo O X.AFLP analysis of Guadua angustifolia (Poaceae:Bambusoideae) in Colombia with emphasis on the Coffee Region[J].Bamboo Science and Culture,2002,16(1):32-42 [8] 胖铁良,郭晓军,李璐滨,等.利用AFLP分子标记技术探讨部分竹类植物属间亲缘关系[J].植物生理与分子生物学研究,2009,280-284 [9] Doyle J J, Doyle D J. Isolation of plant DNA from fresh tissue[J].Focus,1990,12:13-15 [10] 李潞滨,郭晓军,彭镇华,等.AFLP引物组合数量对准确研究竹子系统关系的影响[J].植物学通报,2008,25(4):449-454 [11] 卢江杰,郑 蓉,杨 萍,等.利用AFLP技术研究不同产地绿竹的遗传多样性[J].世界竹藤通讯,2008,6(5):10-14 [12] Nei M. Molecular Population Genetics and Evolution[M]. Amsterdam and New York: North Holland, 1973 [13] Rohf F J.NTSYS—pc.Numerical Taxonomy and Multivariate Analysis System,Version 2.1[M].Exeter Software, Setauket, New York: 2000 [14] Nei M.Estimation of average heterozygosity and genetic distance from a small number of individual[J].Genetics,1978,89:583-590 [15] Arise J C, Hamrick J L. Conservation Genetics, Case Histories from Nature[M]. Chapman& Hal1,New York:1996:189-190 [16] 魏 瑜,毛竹等36种竹类植物的RAPD分析 .福州:福建师范大学,2005 [17] 李 鹏,杜 凡,普晓兰,等.巨龙竹种下不同变异类型的RAPD分析[J].云南植物研究,2004,26(3):290-296 [18] Zawko G,Kranss S L,Dixon K W,et al..Conservation genetics of the rare and endangered Leueopogon obtectus(Ericaeeae)[J]. Moleculer Ecology,2001,10(10):2389-2396 [19] Slatkin M. Gene flow and the geographic structure of natural populations [J].Science, 1987, 236 (4803):787-792 [20] Wirght S.The genetic structure of populations[J].Ann Eugen,1951,15:323-354
计量
- 文章访问数: 3259
- HTML全文浏览量: 198
- PDF下载量: 1864
- 被引次数: 0