Abstract: The bioacoustic signals often work alone or together with other signals like visual or olfactory signals in insect communication. The adult stridulatory signals of red turpentine beetle (RTB), Dendroctonus valens, were recorded and stored digitally after amplification in three experiments (glass phloem sandwich rearing, bolt rearing and directly field recording). The signals in different behavior were then analyzed by a specific acoustic software Adobe Audition and Matlab for echeme, duration, frequency, time domain and frequency domain analysis. The stridulatory apparatus of both male and female were observed by scanning electron microscope. The result showed that the frequency of the stridulatory sound by D. valens was from 3 kHz to 5.3 kHz, and there were clearly differences between the males and females, both in the sounds frequency and in apparatus structure. There were slightly differences with the signals recorded in different behavior, which proved the signals played a role in RTB behavior.