蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证

周静; 张炜; 李大明; 张锡九; 陈其兵

蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证

1.
四川农业大学风景园林学院, 四川成都 611130
2.
四川师范大学生命科学学院, 四川成都 610066
3.
四川省林业科学研究院, 四川成都 610081
基金项目:
四川省国际合作与交流项目(2012HH0039)
中图分类号: S718.46

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

1.
College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2.
College of Life Science, Sichuan Normal University, Chengdu 610066, Sichuan, China
3.
Sichuan Academy of Forestry, Chengdu 610081, Sichuan, China
CLC number: S718.46

摘要: CBF 类转录因子是调控植物冷害应答反应的关键因子。从蓝花楹中克隆到一个457 bp CBF 类基因片段,序列分析表明其编码142 氨基酸的片段,并与多个物种CBF1 基因序列高度同源,且具有CBF 家族的AP2 核心结构域,因此将其命名为JaCBF1。原核表达的结果显示此基因片段编码16 kDa 的蛋白,表达分析结果显示JaCBF1于低温条件下在蓝花楹根、茎、叶中有差异性转录。Southern 杂交结果揭示JaCBF1 在蓝花楹基因组中存在至少2 个拷贝。通过脓杆菌介导方法将JaCBF1异源转录到拟南芥中,荧光共聚焦显微照相结果显示其编码的蛋白质定位于细胞核,冷处理实验发现转基因拟南芥植株的耐冷性得到了显著提高,表明此新片段具有CBF 类植物耐冷因子的典型功能。
- 蓝花楹
- / CBF
- / 转录因子
- / 诱导型表达
- / 耐寒性功能
Abstract: CBFs (CRT/DRE-binding factor) are key transcription factors regulating the chilling response in plants. A 457 bp CBF-like fragment was cloned from Jacaranda acutifolia. Sequence analysis showed that the fragment (named as JaCBF1) encoded a protein of 142 amino acids, and was highly homologous to many known CBF1-like transcription factors with the AP2 core domain that belongs to CBF gene family. The recombinant plasmid containing JaCBF1 sequence was introduced into Escherichia coli. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that JaCBF1 was translated into a 16 KDa protein. Expression pattern analysis indicated that JaCBF1 could be induced with different patterns and degrees by low temperature in roots, stems, and leaves of J. acutifolia. Southern blot revealed that the J. acutifolia genome contained at least two copies of JaCBF1. Ectopic transcription in Arabidopsis thaliana revealed that JaCBF1 functioned in the nuclei, and its expression could significantly improve the freezing resistance of transgenic A. thaliana. The results of study suggest that the novel JaCBF1 is a typical CBF-like transcription factor, and plays an important role responding to cold stress in J.acutifolia.
- Jacaranda acutifolia
- / CBF
- / transcription factor
- / induced expression
- / chilling resistance

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蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

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蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证

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Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

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蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

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蓝花楹JaCBF1转录因子片段的序列分析及耐寒性功能验证

English Abstract

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

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