桃果实醇酰基转移酶基因的克隆及对外源乙烯的响应表达

王贵章; 陈新; 赵天田; 梁丽松; 马庆华; 王贵禧

桃果实醇酰基转移酶基因的克隆及对外源乙烯的响应表达

1.
林木遗传育种国家重点实验室, 国家林业局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091
2.
山东省果树研究所, 山东省果树生物技术育种重点实验室, 山东泰安 271000
基金项目:
国家自然科学基金资助项目（31071834）
中图分类号: S662.1

Cloning, Characterization and Expression of Alcohol Acyltransferase Gene which Responses to Exogenous Ethylene in Peach Fruit

1.
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2.
Shandong Institute of Pomology, Shandong Provincial Key Laboratory of Fruit Tree Biotechnology Breeding, Taian 271000, Shandong, China
CLC number: S662.1

摘要: 为研究桃果实酯类香气的生物合成，克隆了桃醇酰基转移酶基因AAT，并研究了外源乙烯对该基因mRNA转录水平的影响。根据桃基因组测序结果，采用RACE和反转录PCR技术克隆到1个与醇酰基转移酶基因同源的cDNA，序列全长1 486 bp，CDS区长度为1 353 bp，编码450个氨基酸，命名为PpAAT2。PpAAT2蛋白属于PLN02481超级家族，含有植物转移酶基因2个高度保守结构域HXXXD和D（N）F（V）GWG。基因位于桃第5条染色体，BAC注释为1条包含1个内含子的mRNA序列转录链，CDS区有2处碱基突变，其中，555 bp处碱基由C突变为G，导致丙氨酸突变为缬氨酸。AAT基因响应乙烯表达，0 ℃冷藏桃施加外源乙烯促进AAT基因表达水平；该基因在叶片、花和成熟果肉中均表达，且在叶片和花中的表达丰度比果实高，推测其在不同的生理过程中均发挥作用。
- 桃果实
- / 乙醇酰基转移酶基因
- / 酯类
- / 外源乙烯
Abstract: This research cloned and analyzed the alcohol acyl-transferase gene AAT from peach, and detected the response of the gene to exogenous ethylene. Based on the peach genome published (Genome ID: ppa005625m), the specific primers were designed and the full-length of the gene was cloned using RACE PCR. The content of ester volatiles were determined by GC-MS from the storage peach fruits. The full-length of AAT was obtained with 1 486 bp and the open reading frame (ORF) was 1 353 bp. Bioinformation analysis indicated that this gene encoded 450 amino acids, containing three conserved domains LXXYYPLAGR, HXXXD and D(N)F(V)GWG of plant transferase gene, which belonging to PLN02481 super family. The gene was named as PpAAT2 and mapped in the 5th peach chromosome. BAC annotation presented that the gene was the mRNA transcription chain containing 2 exons separated by 1 introns with 3 447 bp. C mutated to G in PpAAT2 on seq 555 bp, resulting alanine to valine. PpAAT2 gene expressed in response to exogenous ethylene which promoted PpAAT2 gene expression level in 0 ℃ cold-stroage peach fruits. PpAAT2 gene expressed in leaves, flowers and ripe flesh, suggesting that the gene played a role in various physiological processes.
- peach fruit
- / alcohol acyl-transferase gene (AAT)
- / esters
- / exogenous ethylene

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桃果实醇酰基转移酶基因的克隆及对外源乙烯的响应表达

Cloning, Characterization and Expression of Alcohol Acyltransferase Gene which Responses to Exogenous Ethylene in Peach Fruit

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桃果实醇酰基转移酶基因的克隆及对外源乙烯的响应表达

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Cloning, Characterization and Expression of Alcohol Acyltransferase Gene which Responses to Exogenous Ethylene in Peach Fruit

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桃果实醇酰基转移酶基因的克隆及对外源乙烯的响应表达

Cloning, Characterization and Expression of Alcohol Acyltransferase Gene which Responses to Exogenous Ethylene in Peach Fruit

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桃果实醇酰基转移酶基因的克隆及对外源乙烯的响应表达

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Cloning, Characterization and Expression of Alcohol Acyltransferase Gene which Responses to Exogenous Ethylene in Peach Fruit

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