Abstract: This study is to determine the optimum receptive period of the stigma, the feasible pollination method, the suitable pollination environment for the cross breeding of Corylus. Taking C. kweichowensis as experiment material, C. heterophylla and C. heterophylla × C. avellana as control groups, the female inflorescences were pollinated with mixed pollen of C. kweichowensis, C. heterophylla and C. heterophylla × C. avellana at different blooming periods, using different pollination methods and under different environments. The pollinated female inflorescences were excised at different time, the pollen adhesion number, germination rate and the pollen tube were observed by fluorescent microscopy. The results showed that the descending order of the stigma receptivity of C. kweichowensis in different blooming periods was florescence period > terminal florescence period > red dot period. The stigma receptivity of loose inflorescences was higher than that of the straight cluster inflorescences. The method of parallel brush pollination was more efficient than the method of point pollination. When the pollinated inflorescences were incubated in a petri dish with double-layer wet filter paper, both the germination and growth speeds of pollen tubes were faster than that in the greenhouse and the field, indicating that humid and mild environment was more suitable for the adhesion and germination of the pollen, as well as the pollen tube growth of C. kweichowensis. The temperature and humidity and the germplasm of the pollinated inflorescences had important effects on the germination of the pollen. The speed of pollen tube growth was strongly influenced by environmental temperature and humidity, but had nothing to do with germplasm.