植物生长调节剂对油茶芽苗砧嫁接愈合的影响
Effect of Plant Growth Regulators on the Healing of the Nurse Seed Grafted Unions of Camellia oleifera
-
摘要: 采用正交试验设计,通过制作石蜡切片以及光学显微观察系统研究了植物生长调节剂对油茶芽苗砧嫁接愈合及嫁接苗成活的影响。结果表明:GGR6、IBA、芸苔素内酯能够明显促进愈伤组织的分化形成、砧木和穗条愈伤组织的连接与延伸,进而加快嫁接苗的愈合过程,各处理愈伤比例均值较对照的增幅为28.01%,且比对照至少提前15 d完成愈合;GGR6、IBA、芸苔素内酯的不同浓度及不同施用方法浸基质、蘸根、喷叶对愈伤比例的形成以及嫁接苗成活率的影响差异极显著。促进油茶芽苗砧嫁接愈合的最佳处理方式为:0.03 g·L-1 GGR6、0.2 g·L-1 IBA及0.140.28 mL·L-1 芸苔素内酯混合液500 mL+浸基质1.42 L。Abstract: In order to study the effect of plant growth regulators on the healing progress of the nurse seed grafted unions of Camellia oleifera, an orthogonal experiment of GGR6, IBA, Brassinolide, different concentration and application method (leaching medium, dunking root, and spraying leaves) on the nurse seed grafted seedlings of C. oleifera was carried out. The paraffin sections and microscopic observation system were used to measure the area of the callus and grafted union and get the healing rate, then the healing progress was made to be measurable. The results showed that the application of plant growth regulators significantly promoted the healing progress, the form of the callus and the connection of stock and scion. The average healing rate of different treatment was 28.01% higher than that of the control group, and the time required for the grafted union to be healed was 15 days less than the control group. The effect of different concentrations and application methods of plant growth regulators on the healing progress and survival rate was significantly different, among these treatments, the best was 0.03 g·L-1 GGR6, 0.2 g·L-1 IBA and 0.14 mL·L-1 0.28 mL·L-1 Brassinolide mixed liquid to leach medium 1.42 L per 500 mL.
-
Key words:
- plant growth regulator
- / Camellia oleifera
- / nurse seed grafting
- / healing progress
- / survival rate
-
[1] 庄瑞林.中国油茶[M].2版.北京:中国林业出版社,2008:23-29. [2] 韩宁林,赵学民.油茶高产品种栽培[M].北京:中国农业出版社,2009:10-15. [3] Fernández-garcía N, Carvajal M, Olmos E. Graft union formation in tomato plants: Peroxidase and catalase involvement[J]. Annals of Botany,2004,93(1):53-60. [4] 杨世杰.嫁接过程的组织学和细胞学观察[J].北京农业大学学报,1987,13(3):359-366. [5] 张红梅,丁 明,姜 武,等.不同苗龄接穗西瓜嫁接体愈合的组织细胞学研究[J].园艺学报,2009, 39(3):493-500. [6] 冯金玲,杨志坚,陈 辉.油茶芽苗砧嫁接口不同发育时期差异蛋白质分析[J].应用生态学报,2012,23(8): 2055-2061. [7] 冯金玲,杨志坚,陈 辉,等.油茶芽苗砧嫁接体的亲和性生理[J].福建农林大学学报:自然科学版,2011,40(1):24-30. [8] 刘传荷,洪 健,夏国华,等.山核桃嫁接过程中愈伤反应的细胞学观察[J].林业科学,2009,45(6):34-40. [9] Ermel F F, Poëssel J L, Faurobert M, et al. Early scion/stock junction in compatible and incompatible pear/pear and pear/quince grafts: A histo-cytological study[J]. Annals of Botany, 1997,79(5):505-515. [10] 朱 进,别之龙,徐 荣,等.不同砧木嫁接对黄瓜生长、产量和品质的影响[J].华中农业大学学报,2006, 25(6): 688-671. [11] 杨世杰,娄成后.嫁接隔离层两侧愈伤组织中的壁傍体[J].植物学报,1988,30(5):480-484. [12] 丁平海,沙立杰,郗荣庭.核桃苗木枝接愈合过程观察[J].河北农业大学学报,1986,9(4):6-11. [13] 张新忠,章德明,张建阁,等.矮砧及乔砧苹果树嫁接口的解剖观察[J].园艺学报,1995,22(2):177-122. [14] 卢善发,唐定台,宋经元,等.利用植物激素调控嫁接形成的初步研究[J].植物学报,1996,38(4):307-311. [15] 杨志坚,冯金玲,陈 辉.油茶芽苗砧嫁接口愈合过程解剖学研究[J].植物科学学报,2013,31(3):313-320. [16] 郑碧娟,苏丽芳,陈世品,等.外源激动素对油茶芽苗砧嵌合体愈合的效应[J].福建农林大学学报:自然科学版,2014,43(2):146-150. [17] 苏丽芳,林文俊,陈世品,等.外源IAA对油茶芽苗砧嵌合体愈合的效应[J].福建林业科技,2012,39(3):57-62. [18] 丁海平,郗荣庭.核桃枝接愈合过程的解剖学观察[J].林业科学,1991,27(4):457-461. [19] 李和平.植物显微技术[M].2版.北京:科学出版社,2009:9-47. [20] 李正理.植物组织制片学[M].北京:北京大学出版社,1996:10-30. [21] 张学娟,陈玉琼.藤茶的显微结构观察[J].华中农业大学学报,2011,30(6):685-690. [22] 王 瑞,陈永忠,王湘南,等.油茶芽苗砧嫁接愈合过程中砧木和穗条相关生理指标的研究[J].西北农林科技大学学报:自然科学版,2014,42(1):46-50. [23] 周 华,董凤祥,曹炎生,等.核桃子苗砧嫁接及相关生理指标的研究[J].林业科学研究,2007,20(1):53-57. [24] 张晋元,张宏平,姬爱国.园艺植物嫁接过程愈伤组织微观研究进展[J].农业工程,2013,3(6):154-156. -
点击查看大图
计量
- 文章访问数: 3116
- HTML全文浏览量: 235
- PDF下载量: 857
- 被引次数: 0
下载:
