不同砧木嫁接甜柿苗期生长生理特性及亲和性评价
Evaluation on Affinity, Growth Trait and Physiology Indicators of Persimmon Grafted on Different Stocks
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摘要: 以浙江柿、君迁子、油柿及537号和514号2种不同柿品种为砧木的‘阳丰’甜柿嫁接苗为试材,对不同砧木的嫁接苗高生长、抗氧化物酶活性、丙二醛(MDA)含量及叶绿素含量等指标的年动态变化进行观测分析,初步评价不同砧木的甜柿嫁接苗苗期亲和性差异。结果表明:(1)以浙江柿、柿537号、柿514号为砧木的甜柿嫁接苗生长速率高于以油柿和君迁子为砧木的嫁接苗;(2)以油柿为砧木嫁接苗的叶绿素及可溶性蛋白含量最低,嫁接苗出现提前衰老的现象;(3)以油柿为砧木的嫁接苗MDA含量均高于其余4种嫁接苗,在生长后期表现更明显;而以油柿为砧木的嫁接苗抗氧化物酶活性在生长前期低于其余4种嫁接苗,在生长后期高于其余4种嫁接苗,砧穗间相互适应性较弱;(4)根据各指标的年动态变化,结合生长及生理指标的主成分分析和隶属函数法分析,得出5种不同砧木的甜柿嫁接苗苗期亲和性强弱顺序:浙江柿>柿537号>柿514号>君迁子>油柿。浙江柿、柿537号、柿514号适宜作‘阳丰’甜柿的砧木,而油柿不宜作‘阳丰’甜柿的砧木。Abstract: Taking ‘PCNA Youhou’ persimmon grafted on the root-stocks of Diospyros glaucifolia, D. lotus, D. oleifera, D. kaki of ‘537’,‘514’ As test material, the annual dynamic change of growth, activity of membrane lipid antioxidant enzymes, content of malomdiadehyde and chlorophyll of different grafted persimmon during seedling period were studied. The results were used to evaluate the graft compatibility of different grafted persimmon. It was indicated that:(1) The growth rate of D. glaucifolia, D. kaki of ‘537’,‘514’ grafted seedlings was higher than that of D. lotus, D. oleifera grafted seedlings. (2) The contents of chlorophyll and soluble protein were the least in D. oleifera grafted seedlings and it tended to show early aging.(3) The contents of malomdiadehyde was higher than that of the other grafted seedlings, especially when the root-stocks was D. oleifera during the later stage of growth. While its activity of antioxidant enzymes was lower than that of the others in the early growth stage and higher during the later stage of growth, and the adaptation between stocks and scion was weak. (4) Analyzing the growth and physiology indicators of the grafted persimmon by principal component and membership function analysis, it turned out that the affinity order was: D. glaucifolia >D. kaki of ‘537’ > D. kaki of ‘514’ > D. lotus > D. oleifera. Combining the annual dynamic change of indicators, the results showed that the D. glaucifolia, D. kaki of ‘537’, and ‘514’ were suitable to ‘PCNA Youhou’, while the D. oleifera was not.
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Key words:
- root-stock
- / malomdiadehyde
- / persimmon
- / growth trait
- / antioxidant enzymes
- / chlorophyll
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[1] 王 威,刘 燕.植物嫁接亲和性鉴定研究进展[J].湖北农业科学,2012,51(10):1950-1953. [2] 王 燕,谢 辉,陈利萍.植物嫁接诱导的遗传变异机理的研究进展[J].遗传,2011,33(6):585-590. [3] 卢善发.番茄/番茄嫁接体发育过程中的过氧化物酶同工酶[J].园艺学报,2000,27(5):340-344. [4] 肖 艳,黄建昌,高平飞,等.龙眼砧穗过氧化物酶同工酶及嫁接亲合力初探[J].西南农业大学学报,2001,23(1):70-72. [5] 石雪晖,王淑英,吴艳纯,等.葡萄叶片中生理生化物质含量与嫁接亲和力关系的研究[J].果树学报,2001,18(1):24-27. [6] 杨 瑞.葡萄砧穗组合筛选及嫁接早期亲和力鉴定[D].兰州:甘肃农业大学,2007. [7] Musacchi S,Pagliuca G,Kindt M,et al.Flavonoids as markers for pear-quince graft incompatibility[J].J Appl Bot,2000, 74(5/6):206-211. [8] Errea P,Gutmann M,Feucht W.Physiological implications of flavan-3-ols in apricot-rootstock combinations[J].Adv Hort Sci,2000,14(3):126-134. [9] 武季玲.葡萄品种嫁接亲和力研究[D].兰州:甘肃农业大学,2001. [10] 宋红苗.影响美国黑核桃嫁接成活率的生理生化特性分析及初步选优[D].太原:山西农业大学,2006. [11] 龚榜初,王劲风.柿不同砧木生物学特性的研究[J].经济林研究,1997,15(1):9-12. [12] 刘 勇,霍光华,刘善军,等.不同砧木"富有"甜柿幼树生长及叶片生理生化特性研究[J].江西农业大学学报,2000,22(1):54-57. [13] 欧 毅,王玉霞,王 进,等.砧木对甜柿光合效能和生理生化指标的影响[J].西南园艺,2004,32(5):4-6. [14] 王元裕,李伯均,周碧英,等.甜柿砧穗组合嫁接亲和力研究[J].园艺学报,1996,23(2):110-114. [15] 高志红,章 镇,段 锋.甜柿砧、穗及其接合部位解剖结构观察[J].江苏林业科技,1996,23(3):21-24. [16] 王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. [17] 汪家政,范明.蛋白质技术手册[M].北京:科学出版社,2007.77-108. [18] 张志良,瞿伟菁.植物生理学实验指导(第三版)[M].北京:高等教育出版社,2003,127-278. [19] 张小红,赵依杰,林 强,等.不同砧木嫁接对无籽西瓜部分生理指标的影响[J].热带作物学报,2012,33(5):832-836. [20] 赵依杰,陈清西,吴宇芬,等.砧木对小型嫁接西瓜生理生化的影响[J].中国农学通报,2008,24(6):319-323. [21] 吴宇芬,赵依杰.不同南瓜砧木对嫁接薄皮甜瓜生理的影响[J].中国农学通报,2007,23(11):253-256. [22] 杨冬冬,黄丹枫.西瓜嫁接体发育中木质素合成及代谢相关酶活性的变化[J].西北植物学报,2006,26(2):290-294. [23] 陈 红,王永清,袁 媛,等.茄子/番茄嫁接体发育过程中的蛋白质含量、POD、CAT和SOD活性及其同工酶研究[J].四川农业大学学报,2006,24(2):144-147. [24] 孙华丽,宋健坤,李鼎立,等.梨不同嫁接组合嫁接愈合过程中生理动态变化研究[J].北方园艺,2013(16): 25-29. [25] 曲云峰,赵 忠,张小鹏.大扁杏嫁接愈合过程中几种生化物质含量的变化[J].西北农林科技大学学报,2008,36(3):74-78. [26] 尹永强,胡建斌,邓明军.植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J].中国农学通报,2007,23(1):105-110. -
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