红松4个组织的转录组数据分析与次生代谢产物的表达差异初探

张振; 张含国; 周宇; 张磊; 于宏影; 张莉

红松4个组织的转录组数据分析与次生代谢产物的表达差异初探

1.
东北林业大学林木遗传育种国家重点实验室, 黑龙江哈尔滨 150040
基金项目:
林业公益性行业科研专项"红松果材兼用林良种选育与定向培育技术研究"(201204320)
中图分类号: S791.245

Differential Gene Expression Analysis on Secondary Metabolites and Transcriptome Sequencing to Four Tissues of Pinus koraiensis

1.
State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China
CLC number: S791.245

- 红松
- / 转录组
- / 基因注释
- / 次生代谢途径
Abstract: This research compared the transcriptomes of four different tissues of Pinus koraiensis by using RNA-seq technique and a lot of differentially expressed unigenes were obtained. A total of 21.3 GB of data was obtained and 41 476 expressed unigenes were received after assembling. Through GO and COG classification, the commented and transcribed sequences were classified into 24 or 56 functional categories, which referred to many biochemical processes such as material and energy metabolism, signal transduction, transcriptional regulation and defense reaction. Through pathway enrichment analysis, the pathways associated with grease synthetic fatty acid biosynthetic and terpenoids synthesis were found. Two unigenes of metabolic pathways in KEGG database were compared and 16 key enzyme genes of fatty acid synthesis in other species homologous and 34 key enzyme genes of terpenoid biosynthetic pathway were obtained. By high-throughput sequencing and bioinformatics research, a large number of Pinus koraiensis transcriptome unigenes was obtained. This research provides references for the research on the genes related to secondary metabolites synthesis in Pinus koraiensis.
- Pinus koraiensis
- / transcriptome
- / gene annotation
- / secondary metabolites biosynthesis

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红松4个组织的转录组数据分析与次生代谢产物的表达差异初探

Differential Gene Expression Analysis on Secondary Metabolites and Transcriptome Sequencing to Four Tissues of Pinus koraiensis

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红松4个组织的转录组数据分析与次生代谢产物的表达差异初探

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Differential Gene Expression Analysis on Secondary Metabolites and Transcriptome Sequencing to Four Tissues of Pinus koraiensis

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红松4个组织的转录组数据分析与次生代谢产物的表达差异初探

Differential Gene Expression Analysis on Secondary Metabolites and Transcriptome Sequencing to Four Tissues of Pinus koraiensis

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红松4个组织的转录组数据分析与次生代谢产物的表达差异初探

English Abstract

Differential Gene Expression Analysis on Secondary Metabolites and Transcriptome Sequencing to Four Tissues of Pinus koraiensis

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