烟草和毛白杨次生维管发育相关MYB转录因子分析

郭伟; 赵树堂; 卢孟柱

烟草和毛白杨次生维管发育相关MYB转录因子分析

1.
中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091
基金项目:
"十二·五"863计划课题"林木优质、速生性状调控基因的分离及育种技术研究(2013AA102702)"。
中图分类号: S792.117;S572

Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

1.
Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
CLC number: S792.117;S572

摘要: [目的]为探讨额河杨和银灰杨天然杂种的起源机制,[方法]应用18对SSR标记,从分子水平上对新疆额尔齐斯河流域杨属植物的种间关系进行分析研究。[结果]表明:(1)SSR系统发育树将整个流域天然杨属植物分为两大类群,即黑杨派和青杨派为一类,白杨派为一类;(2)白杨派派内系统聚类图显示,银白杨、欧洲山杨、银灰杨三个树种均有较大的遗传分化,特别是杂种银灰杨似乎更大;(3)黑杨派和青杨派的UPGMA分类图显示,青杨派和黑杨派分属于2个分支,其中,青杨派内部分化相对简单,分为2支,均为典型的苦杨;黑杨派内部的分化较为复杂,可分为4类,包括典型的欧洲黑杨、额河杨和回交子代。[结论]杂种额河杨具有更多的欧洲黑杨的遗传成分,因此,将额河杨放到黑杨派是正确的。
- 烟草
- / 毛白杨
- / MYB
- / 次生生长
Abstract: Tobacco plants have typical secondary vascular development process and can be used to study the function of genes related to secondary vascular development. 48 pairs of MYB orthologous genes obtained from tobacco and poplar were identified by searching in the entire poplar genome sequence with MYB transcription factor obtained from transcriptome of tobacco vascular tissues as probes, and were divided into 23 subgroups. The analysis of gene expression levels obtained using RNA-seq in tobacco and poplar suggested that 15 pairs of MYB orthologous genes shared similar expression patterns both in tobacco and poplar. The results of expression analysis using real time RT-PCR in different tissues of tobacco and poplar indicated that the MYB orthologous genes shared similar expression patterns both in tobacco and poplar. It is thus feasible to rapidly analyze the function of MYB transcription factors in vascular development process with tobacco.
- tobacco
- / Populus tomentosa
- / MYB
- / secondary growth

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烟草和毛白杨次生维管发育相关MYB转录因子分析

Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

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烟草和毛白杨次生维管发育相关MYB转录因子分析

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Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

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烟草和毛白杨次生维管发育相关MYB转录因子分析

Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

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烟草和毛白杨次生维管发育相关MYB转录因子分析

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Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

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目录