大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

林英华; 卢萍; 赵鲁安; 谭飞; 徐演鹏; 贾旭东; 李慧仁; 刘学爽; 韦昌雷; 王立中

大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

1.
中国林业科学研究院湿地研究所, 北京 100091
2.
黑龙江省林业监测规划院, 黑龙江哈尔滨 150080
3.
广西九万山国家级自然保护区, 广西柳州 545300
4.
大兴安岭林业集团公司农林科学研究院, 黑龙江加格达奇 165000
基金项目:
科技部(林业)公益性行业科研专项(编号:201004074),国家自然科学基金项目(31372184)资助。
中图分类号: S714.3

The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

1.
Institute of Wetland Research, Chinese Academy of Forestry, Beijing 100091
2.
Institute of Heilongjiang Provincial Monitoring and Planning of Forestry, Harbin 154080, Heilongjiang
3.
The Administration Bureau of Jiuwan Mountain National Natural Reserve, Liuzhou 545300, Guangxi
4.
Research Institute of Agriculture and Forestry, Greater xing'an Forestry Group, Jiagedaqi 165000, Heilongjiang
CLC number: S714.3

摘要: [目的]研究森林沼泽演替与火干扰条件下土壤微生物结构与多样性变化,为进一步揭示土壤微生物群落在森林沼泽保护与恢复中的作用提供依据。[方法]采用磷脂脂肪酸法与BIOLOG方法,研究大兴安岭南瓮河国家自然保护区内主要森林沼泽类型(兴安落叶松-狭叶杜香-藓类沼泽、兴安落叶松-兴安杜鹃-藓类沼泽、兴安落叶松+白桦-苔草沼泽)与2006年受不同火强度干扰沼泽(重度火烧的兴安落叶松-兴安杜鹃-藓类沼泽和中度火烧的兴安落叶松+白桦-苔草沼泽)土壤微生物群落特征,探讨沼泽主要发育阶段与火干扰强度对土壤微生物群落的影响。[结果]研究区域土壤微生物群落以16:00(16.29±5.62 nmol·g-1)、甲烷氧化菌(18:1ω8t)(9.89±8.61 nmol·g-1)与16:1ω7c(9.79±3.24 nmol·g-1)的微生物为优势种群。土壤微生物总PLFAs含量、革兰氏阳性菌(G+)中a15:0、i16:0、i17:0、革兰氏阴性菌(G-)中的cy19:0、真菌中的18:2ω6c、甲烷氧化菌(18:1ω8t)与森林沼泽发育阶段、火干扰明显相关(pp+/G-)未随着沼泽发育呈现出规律性变化,其比值受火干扰后明显发生改变(ppα-D-Lactose =2.87 p=0.080,FL-Threonine=3.00 p=0.078),土壤真菌对D-Mannitol、D-glucosaminic Acid利用存在差异性(FTween 80=2.75,p=0.088, FD-Mannitol=3.53 p=0.047, FD-glucosaminic Acid=4.67 p=0.022),但沼泽类型与火干扰未对土壤微生物功能多样性产生影响(p>0.05)。[结论]土壤微生物量与沼泽发育阶段相关;沼泽发育与火干扰改变土壤微生物群落结构。土壤细菌与真菌对碳源利用方面具有选择性。
- 磷脂脂肪酸法(PLFAs)
- / BIOLOG 生态板
- / 土壤微生物群落水平生理图谱
- / 土壤性质
- / 典型判别分析
Abstract: [Objective]The aim of this paper was studied the characteristic of soil microbiological community and its diversity at forest marsh development and fire disturbance, to understand the role of soil microbial community in the forest marsh conservation and restoration.[Methods]We used phospholipid fatty acids (PLFAs) to portrait the community composition and community level physiological profiles (CLPP) to describe the functional diversity of the microbial community. [Results]Total of 5 types of marsh were chosen in Natural reserve area of Nanwenhe in Greater xing'an Mountain, which included xing'an Larch-Ledum palustre-moss marsh, xing'an Larch-xing'an azalea-moss marsh, xing'an Larch-birch-carex marsh, and Larch-xing'an azalea-moss marsh by severe fire disturbance, xing'an Larch-birch-carex marsh by mild fire disturbance at 2006. It was found that the PLFAs of 16:00(16.29±5.62 nmol·g-1), 18:1ω8t(9.89±8.61 nmol·g-1)and 16:1ω7c(9.79±3.24 nmol·g-1)were dominance in microbial community, and the dominant species contributed significantly to variations in soil microbial biomass, especially Gram positive bacteria (cy19:0), Fungi(18:2ω6c) and Methane oxidizing bacteria(18:1ω8t). The rate of normal saturated fatty acid and monounsaturated fatty acid increased, and significantly increased after the interference of fire(p(pα-D-Lactose=2.87 p=0.080, FL-Threonine=3.00 p=0.078), and all of Tween 80, D-Mannitol, D-glucosaminic Acid were significantly difference by utilization of soil fungi(FTween 80=2.75 p=0.088, FD-Mannitol =3.53 p=0.047, FD-glucosaminic Acid=4.67 p=0.022),but the functional diversity of microbial community had not been effected by both the type of marsh and fire disturbance. [Conclusion]soil microbial biomass was correlated with the development stage of the marsh, and the soil microbial community structure was changed with the development stage of the marsh and fire disturbance. Soil bacteria and fungi were selective in the use of carbon resource.
- PLFA profiles
- / BIOLOG Eco-Plate
- / community level physiological profiling
- / soil property
- / discriminant function analysis

[1]	Haase J, Brandl R, Scheu S, et al. Above and belowground interactions are mediated by nutrient availability[J]. Ecology, 2008, 89:3072-3081.
[2]	Bartelt-Ryser J, Joshi J, Schmid B, et al. Soil feedbacks of plant diversity on soil microbial communities and subsequent plant growth[J]. Plant Ecology, Evolution and Systematics, 2005,7: 27-49.
[3]	Thoms C, Gattinge A, Jacob M, et al. Direct and indirect effects of tree diversity drive soil microbial diversity in temperate deciduous forest[J]. Soil Biology & Biochemistry,2010,42:1558-1565.
[4]	Fierer N, Strickland M S, Liptzin D, et al. Global patterns in belowground communities[J]. Ecology Letters, 2009, 12: 1238-1249.
[5]	Merila P, Malmivaara-Lamsa M, Spetz P, et al. Soil organic matter quality as a link between microbial community structure and vegetation composition along a successional gradient in a boreal forest[J]. Applied Soil Ecology, 2010, 46: 259-267.
[6]	Cardinale BJ, Duffy JE, Gonzalez A, et al. Biodiversity loss and its impact on humanity[J]. Nature, 2012, 486: 59-67.
[7]	Bardgett R D, van der Putten W H. Belowground biodiversity and ecosystem functioning[J]. Nature, 2014, 515: 505-511.
[8]	Tscherkoa D, Hammesfahr U, Zeltner G, et al. Plant succession and rhizophere microbial communities in recently deglaciated alpine terrain[J]. Basic and Applied Ecology, 2005,6:367-383.
[9]	Andersen R, Grasset L, Thormann M N, et al. Changes in microbial community structure and function following Sphagnum peatland restoration[J]. Soil Biology & Biochemistry, 2010, 42,291-301.
[10]	Vázquez F J, Acea M J, Carballas T. Soil microbial populations after wildfire[J]. FEMS Microbiology Ecology, 1993,13: 93-103.
[11]	Fontúrbel MT, Barreiro A, Vega J A, et al. Effects of an experimental fire and post-fire stabilization treatments on soil microbial communities[J]. Geoderma, 2012,191:51-66.
[12]	白爱芹,傅伯杰,曲来叶,等,大兴安岭火烧迹地恢复初期土壤微生物群落特征[J].生态学报,2012,32(15): 4762-4771.
[13]	郑琼,崔晓阳,邸雪颖,等.不同林火强度对大兴安岭偃松林土壤微生物功能多样性的影响[J].林业科学,2012,48(5)95-100.
[14]	中国湿地植被编写委员会.《中国湿地植被》[S].北京:科学出版社1999.
[15]	Frostegård A, Bååth E, Tunlid A. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty-acid analysis[J]. Soil Biology & Biochemistry, 1993, 25: 723-730.
[16]	Bååth E. The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi[J]. Microbial Ecology, 2003, 45: 373-383.
[17]	Frostegård Å, Tunlid A, Bååth E. Use and misuse of PLFA measurements in soils[J]. Soil Biology & Biochemistry,2011,43, 1621-1625.
[18]	Balser TC, Wixon D. Investigating biological control over soil carbon temperature sensitivity[J]. Global Change Biology, 2009,15:2935-2949.
[19]	甘秋妹,孙海龙,郑红,等.大兴安岭不同退化阶段土壤和植物C、N、P浓度及其化学计量特征[J].森林工程,2013,29(3): 1-5.
[20]	刘银良,闫敏华,孟宪明, 等.大兴安岭森林火灾对沼泽土壤的影响[J].地理科学,1995,15(4):378-384.
[21]	Joergensen R G, Wichern F. Quantitative assessment of the fungal contribution to microbial tissue in soil[J]. Soil Biology & Biochemistry, 2008, 40: 2977-2991.
[22]	Carrasco L, Gattinger A, Flieβbach A, et al. Estimation by PLFA of microbial community structure associated with the rhizosphere of Lygeum spartum and Piptatherwn miliaceum growing in semiarid mine tailings[J]. Microbial Ecology, 2010,60: 265-271.
[23]	Fang J, Barcelona M J, Alvarez P J. A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification[J]. Soil Biology and Biochemistry, 2000, 31:881-887.
[24]	Thiet R K, Frey S D, Six J. Do growth yield efficiencies differ between soil microbial community differing in fungal: bacterial ratios? Reality check and methodological issue[J]. Soil Biology and Biochemistry, 2006,38: 837-844.
[25]	Vries de F T, Hoffland E, Van Eekeren N, et al. Fungal/bacterial ratios in grasslands with contrasting nitrogen management[J]. Soil Biology and Biochemistry, 2006, 38:2092-2103.
[26]	Diedhiou S,Dossa E L,Badiane A N,et al.Decomposition and spatial microbial heterogeneity associated with native shrubs in soils of agroecosystems in semi-arid Senegal[J]. Pedobiologia, 2009,52: 273-286.
[27]	张莉,党军,刘伟,等.高寒草甸连续围封与施肥对土壤微生物群落结构的影响[J].应用生态学报,2012,23 (11):3072-3078.
[28]	Marschner P, Kandeler E, Marschner B. Structure and function of the soil microbial community in a long-term fertilizer experiment[J]. Soil Biology and Biochemistry, 2003, 35: 453-461.
[29]	Opelt K , Berg C, Schönmann S, Eberl L, Berg G. High specificity but contrasting biodiversity of Sphagnum-associated bacterial and plant communities in bog ecosystems independent of the geographical region[J]. The ISME Journal, 2007, 1, 502-516.
[30]	Preston M D, Smemo K A, McLaughlin J W, et al. Peatland microbial communities and decomposition processes in the James Bay Lowlands, Canada[J]. Frontiers in Microbiology, 2012,3,1-15.
[31]	Pratt B, Riesen R,Johnston C G. PLFA Analyses of Microbial Communities Associated with PAH-Contaminated Riverbank Sediment[J]. Microbial Ecology, 2012,64:680-691.
[32]	丁维新,蔡祖聪.土壤甲烷氧化菌及水分状况对其活性的影响[J].中国生态农业学报, 2003,11(1):94-97.
[33]	倪永清,史学伟,郑晓吉,等.冻土甲烷循环微生物群落及其对全球变化的响应[J].生态学报,2011,31(13):3846-3855.
[34]	Hebel C L, Smith J E, Jr Cromack K. Invasive plant species and soil microbial response to wildfire burn severity in the Cascade Range of Oregon[J]. Applied Soil Ecology, 2009, 42:150-159.
[35]	Wang M, Qu L Y, Ma K M, et al. Soil microbial properties under different vegetation types on Mountain Han[J]. Science China Life Sciences, 2013, 56: 561-570.

[1]	. 昆明金殿林区云南松次生林健康状况与\n土壤相关性分析. 林业科学研究, 2009, 22(6): 865-871.
[2]	盛炜彤 , 杨承栋 , 范少辉 . 杉木人工林的土壤性质变化. 林业科学研究, 2003, 16(4): 377-385.
[3]	杨承栋 , 焦如珍 , 屠星南 , 熊有强 , 陈仲庐 . 杉木林下植被对5～15cm土壤性质的改良*. 林业科学研究, 1995, 8(5): 514-519.
[4]	杨曾奖 , 郑海水 , 周再知 . 胶园间种对枯落物、腐殖质和土壤性质的影响. 林业科学研究, 1996, 9(4): 354-358.
[5]	杨承栋 , 焦如珍 , 盛炜彤 , 夏良放 , 熊有强 , 曾满生 . 江西省大岗山湿地松林土壤性质的变化. 林业科学研究, 1999, 12(4): 392-397.
[6]	张春雨 , 赵秀海 , 郑景明 . 长白山阔叶红松林林隙与林下土壤性质对比研究. 林业科学研究, 2006, 19(3): 347-352.
[7]	高成杰 , 唐国勇 , 刘方炎 , 张春华 , 孙永玉 , 李昆 . 林分结构调整对云南松次生林生长和土壤性质的影响. 林业科学研究, 2017, 30(5): 841-847. doi: 10.13275/j.cnki.lykxyj.2017.05.018
[8]	赵京京 , 王超群 , 董玉红 , 厚凌宇 , 焦如珍 . 细菌肥料对湿地松幼龄林生长及土壤性质的影响. 林业科学研究, 2019, 32(1): 153-159. doi: 10.13275/j.cnki.lykxyj.2019.01.021
[9]	唐艳龙 , 杨清培 , 温小遂 , 王丽娜 , 何小龙 , 余林 . 萧氏松茎象发生与湿地松林地枯落物及土壤物理性质的关系. 林业科学研究, 2010, 23(4): 554-559.
[10]	王宏星 , 孙晓梅 , 陈东升 , 沈亚洲 , 马建伟 . 甘肃小陇山日本落叶松人工林不同发育阶段土壤理化性质的变化. 林业科学研究, 2012, 25(3): 294-301.
[11]	骆土寿 , 刘伟钦 , 尹光天 , 罗瑞强 , 李意德 , 陈德祥 . 顺德森林改造区不同林分土壤环境质量研究. 林业科学研究, 2004, 17(4): 441-446.
[12]	程诗明 , 顾万春 . 苦楝表型区划的研究. 林业科学研究, 2006, 19(3): 337-341.
[13]	牛利敏 , 苗倞婧 , 彭定聪 , 徐秋芳 , 邬奇峰 , 秦华 . 长期粗放经营毛竹林土壤微生物群落演变特征. 林业科学研究, 2017, 30(2): 285-292. doi: 10.13275/j.cnki.lykxyj.2017.02.014
[14]	张青青 , 董醇波 , 邵秋雨 , 陆莹霞 , 董旋 , 梁宗琦 , 韩燕峰 . 杜仲种子内生微生物群落组成及生态功能分析. 林业科学研究, 2023, 36(2): 50-60. doi: 10.12403/j.1001-1498.20220239
[15]	禹飞 , 梁俊峰 . 玫瑰红菇和灰肉红菇对根际土壤微生物群落结构的影响. 林业科学研究, 2022, 35(6): 52-63. doi: 10.13275/j.cnki.lykxyj.2022.006.006
[16]	汪运祥 , 吴航湦 , 何冬梅 , 苏仪 , 王子杨 , 潘龙 , 廖晓丽 , 靳少非 , 郑德祥 . 郭岩山自然保护区丝栗栲天然林分土壤微生物群落沿海拔梯度变化. 林业科学研究, 2023, 36(6): 134-143. doi: 10.12403/j.1001-1498.20230194
[17]	陈步峰 . 用逐步判别分析法对海南岛林业气候区的划分. 林业科学研究, 1990, 3(4): 382-387.
[18]	刘春华 , 吴东梅 , 刘雨晖 , 陈辉 , 沈宝贵 , 蒋宗垲 , 刘小飞 . 氮沉降对米槠天然林土壤有机碳及微生物群落结构的影响. 林业科学研究, 2021, 34(2): 42-49. doi: 10.13275/j.cnki.lykxyj.2021.02.005
[19]	张雁 , 郭同斌 , 潘惠新 , 黄敏仁 , 王明庥 , 诸葛强 . 转Bt基因南林895杨抗虫性及对土壤微生物影响分析. 林业科学研究, 2012, 25(3): 346-350.
[20]	. 海南4种典型林分土壤化学性质比较研究. 林业科学研究, 2009, 22(1): -.

点击查看大图

计量

文章访问数: 3172
HTML全文浏览量: 200
PDF下载量: 1096
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

计量

大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

English Abstract

The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

全文HTML

目录

留言板

大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

计量

出版历程

大兴安岭森林沼泽类型与火干扰对土壤微生物群落影响

English Abstract

The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

全文HTML

目录