Abstract: [Objective] To realize optimal visual adjustment of real stand structure based on the modeling of 3D stand scenes, which adopt the technology of visual simulation. [Methods] Taking the Cunninghamia lanceolata and Liriodendron chinense mixed plantation planted in the Huangfengqiao State-owned Forest Farm, Youxian County, Hu'nan Province were used as samples, the virtual simulation adjustment system was developed by virtual simulation technique, based on the 3D virtual environment. By calculating and analysis of stand angle scale, size ratio, mingling, open index and competition index, the simulated process of adjustment the stand structure in 3D stand scene based on the bulletin board display technology, ray query algorithm and MOGRE, was implemented. [Results] After adjustment of the stand, the average heights of tree changed from 12.8 m to 13.6 m, DBH changed from 17.1 cm to 18.6 cm; the average angle scale from 0.449 to 0.481. The distribution pattern of the stand changed to random pattern from average one. The growth of the stand had a huge advantage as the average size of the stand became 0.519 from 0.511. The average mixture degree of the stand changed to 0.129. After apply the adjusted methods, the stand structure became more stable, the quality of the stand improved a lot. [Conclusion] The visual simulate technology and field investigation data were applied to from the virtual 3D forestry environment. In the 3D scenes, forest managers can not only view the property of every single tree in the stand, but also be able to observe the whole state of the stand. The optimal adjustment of stand structure in virtual forest environment is realized.