A STUDY ON CULTURE OF PANAX GINSENG TISSUE AND CELL I.EFFECTS OF CULTURAL CONDITIONS ON GROWTH OF PAN AX GINSENG CALLUS

Callus was induced from Panax ginseng seed,primary root,young stem and leaf.Effects of temperature,illumination,carbon sources,organic nitrogen sources and plant hormone on its growth were studied.The callus induced from young stem is the best cultural material.The optimum temperature of culture is at 20-24℃,and the optimum illumination is at 2000 lx,and the best carbon source is 3% sugar added with 0.5% glucose.Organic nitrogen source is not necessary for growth of callus and suspension cell in Panax ginseng,and beef extract is injurious to Panax ginseng callus.2,4-D is a necessary hormone for growth of callus and suspension cell,and the optimum concentration is at 0.5-1.0mg/L.The fresh weight of callus increased 4-6 times in solid-gass phase culture for 6 weeks,and the fresh weight of suspension cultural cell increased 100 times for 20 days.