Changes of Soluble Protein Content in Acacia mearnsii Suspension-cultured Cells during Cold Acclimation

Cell suspension cultures of Acacia mearnsii De Wild maintained at 2℃,8 h/d light for 14 days had much more freezing tolerance,and its LT50 was lower from -3.4℃ to -8.2℃.Cold acclimation induced the increase of soluble protein content,and its time course of increase was in accordance with the development of freezing tolerance.Cycloheximide inhibited both the increase of soluble protein and the development of freezing tolerance.During cold acclimation,if cycloheximide was added at different time,the inhibition effects on development of freezing tolerance were different.These results indicated that development of freezing tolerance was nearing completion at about the tenth day of acclimation period.Exogenous sugar accelerated the time course of increase of soluble protein,however it was ineffective on the increase of final total content of soluble protein.Sugar wasn't a factor for the induction of freezing tolerance in cold acclimation of Acacia mearnsii.