Study on Cell Suspension Culture and lsolation of Protoplfast Of Dendrocalamus membranceus

Root crowns of plantlets and young stems with joint of Dendrocalamus membranceus Munro were cultured on MS agar medium supplemented with 5 mg/L 2, 4-D,0.2mg/L KT and 200 mg/L LH, calli in rapid growth were formed. Then, the calli were transferred to a liquid medium with the same medium composition or with diverse levels of 2, 4-D, the cell suspension culture which consisted maily of cell or cell aqqregates, was established. A great amount of protoplasts were released from mesophyll of sterile seedlings and cell suspension cultures, with protoplast yields 5×105/g FW and 2.5×105/g FW respectively,and viability above 80%.