The Enzymes in the Secretions of Pine Wood Nematode (Bursaphelenchus xylophilus)

After surface sterilization in the antibiotic solution of 0.002% actidion and 0.1% sulphostreptomycete, PWN, Bursaphelenchus xylophilus, were in the buffer of Tris HCl, 0.05M, pH7.2, for shaking incubation at 25℃ for 72 hours.Then the suspension was centrifugated at 1500 rpm for 5 min.The supernatant, in which there were secretions of PWN, was filtered through 0.22 μm millipore and precipitated proteins in 100% of the saturation (NH4)2SO4 solution.The sediments were dialysised in redistilled water and eventually dried to powder by freezing vaccum drier.The powder, which was used to detect enzymes in polyacrylamide electrophoresis gel with the dye staining methods of some enzymes, showed that there were cellulases(E.C.3.2.1.4), protease, peroxidase(E.C.3.2.1.4)and amylase(E.C.3.2.1.1) in the secretions of PWN.It is considered from the results that the enzymes mentioned above not only just the cellulase, but all of them possibly take part in the primary reaction of pine wilt disease caused by the nematode of Bursaphelenchus xylophilus.