Factors Effecting on Tissue Culture of Perennial Ryegrass ( Lolium perenne L.)

Plantlets were regenerated from seed-derived callus of perennial ryegrass (Lolium perenne). The replacements of 2,4-D by dicamba, maltose by sucrose in the callus induction medium significantly improved callus induction frequency and overall plant regeneration ability. From 3 mg·L-1 to 9 mg·L-1, the stepwise increase of the 2,4-D concentration in maltose containing callus induction medium obviously increased callus induction frequency but decreased callus differentiation frequency. The callus induction medium supplemented with two auxins (2,4-D and NAA) was much better than that with 2,4-D individual. Elevating concentrations of casein hydrolysate, proline and glutamine did not improve overall plant regeneration frequency.