Somatic Embryogenesis of Parthenocissus tricuspidata

Calli were induced from immature embryos of Parthenocissus tricuspidata which were cultured in improved B5 medium supplemented with 2.0 mg·L-1 6-BA and 0～4 mg·L-1 2,4-D. The inducing frequency of callus ranged from 0 to 100%.Calli were subcultured in the same base medium with various combinations of 6-BA and 2,4-D. Somatic embryoids occurred from improved B5 medium without any regulators. Results showed that the suitable mediums for embryogenesis and plant regeneration from immature embryos of Parthenocissus tricuspidata were:(1)callus inducement:improved B5+6-BA 2.0 mg·L-1+2,4-D 2.0 mg·L-1;(2) subculture medium:improved B5+6-BA 2.0 mg·L-1+2,4-D 1.0 mg·L-1;(3)embryogenic and germination medium:improved B5.The embryogenic frequency was 33%,the germination frequency of embryoids was 53%,and the survival ratio after transplant was 85%. Embryoides occurred from exterior of callus and developed through the stages of globular embryoid,heart shaped embryoid,tin fish-shaped embryoid and cotyledon differentiation stage embryoid.