Tissue Culture of Zoysia matrella and the Establishment of Asexual System

The paper dealt with the studies on the tissue culture and the establishment of asexual system of Zoysia matrella.Two kinds of asexual system were established successfully based on careful studies on the methods and major factors for induction,differentiation and propagation of tufted shoots and calli.One asexual system was maintained through tufted shoots regeneration,using top buds of rhizome of Zoysia matrella as explants,MS + 6-BA 2mg·L-1 + NAA 0.1 mg·L-1 as the effective medium for adventitious shoot induction and MS + KT 3mg·L-1 + NAA 0.1 mg·L-1 as the suitable subculture medium for adventitious shoot propagation.Another asexual system was maintained through callus induction and differentiation,using the base of tufted shoots as explants,MS + 2,4-D 4 mg·L-1 as the optimum medium for callus induction and MS basic medium as the effective medium for differentiation in callus.And the plantlets got acclimatized well,with the survival rate up to 95%.Establishment of asexual system laid a solid foundation for further studies on breeding by means of somaclonal variation and Agrobacterium-mediated genetic transformation.