A Study on Methods for RNA Isolation from Bamboos

Isolation of high quality RNA from bamboos was complicated by high levels of polyphenols and polysaccharides which bind or co-precipitate with RNA. Using TRIzol, modified guanidine isothiocyanate, modified CTAB, modified hot borated and modified SDS method were investigated. Yield and quality were monitored by UV absorbance (A260/A280 and A260/A230) and by native agarose gel, quality and yield of total RNA of five method extracted were compared. Result showed that RNA extraction using modified SDS method resulted in RNA of high quality from five methods investigated, ratio of A260/A280 was over 1.9, and ratio of A260/A230 was over 1.2, this method produced RNA at 560μg·g-1(FW) with no degradation. Modified SDS method is an efficient and effective procedure for the extraction of high quality and yield RNA from bamboos.