Studies on Sustainable Effects for Helicoverpa armigera Nuclear Polyhedrosis Virus to Host Insects
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1.
Research Institute of Forest Ecology, Environment and Protection, CAF, Key Laboratory of Forestry Protection, State Forestry Administration, Beijing 100091, China
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2.
Center Station of Control and Quarantine of Forest Diseases and Pests, Yinchuan 750001, Ningxia, China
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Received Date:
2005-11-09
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Abstract
Third instars larvae of cotton bollworm,Helicoverpa armigera,were infected with different concentrations of Helicoverpa armigera nucleopolyhedrovirus(HaNPV).Survivors were collected respectively and reared in laboratory room.Mortality rate of the larvae,average weight of the single pupa and pupation rate were observed in parental generation and successive filial generations.The results showed that the virus not only led to death of larvae in parental and folial generation,but also impacted the average weight of the single pupa and pupation rate,especially to the parental and the first folial generation.The experimental data indicated that the virus could weaken the host in folial generations,which could control populations of the host for a long time.Meanwhile,polymerase chain reaction(PCR) amplification of polyhedrin gene sequences demonstrated that the virus was present in eggs and pupae whose parents were infected.This method might provide a better understanding of nucleopolyhedrovirus which could transmit from parental generation to folial generation. The research would play an important role in utilizing the virus effectively to control the pest persistently.
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References
|
[1]
|
冀卫荣, 刘贤谦, 师光禄. 核型多角体病毒杀虫剂的开发[J] . 山西农业大学学报, 1998, 18(4): 302~ 305
|
|
[2]
|
张忠信, 张光裕, 彭辉银, 等. 棉铃虫核型多角体病毒在生态环境中的滞留及作用[J]. 中国病毒学, 1995, 10(1) : 56~ 60
|
|
[3]
|
曲良建, 张永安, 王玉珠, 等. PCR法证实棉铃虫核型多角体病毒对棉铃虫经卵和蛹的垂直传递[J] . 中国生物防治, 2005, 21(1): 45~ 48
|
|
[4]
|
范贤林, 卢美光, 孟香清, 等. 棉铃虫室内饲养技术的改进[J] . 昆虫知识, 2003, 40(1) : 85~ 87
|
|
[5]
|
梁革梅, 谭维嘉, 郭予元. 人工饲养棉铃虫技术的改进[J]. 植物保护, 1999, 25(2) : 15~ 17
|
|
[6]
|
HughesD S, Possee R D, L ing L A, et a.l A ctivation and detectionof a laten t bacu lov iru s resem bl ingMam estra brassicae nuclearpolyh edrosis v iru s inM. brassicae insect [J] . V iro logy, 1993, 194: 608 ~615 |
|
[7]
|
Ch arp ent ier G, Desm arteaux J P, Bellon S, et a,l U til ization of thepolym erase chain react ion in the d iagnosis of nu clear polyhedros is v iru s in fect ion s of gyp sy moth (Lym antria d ispar, Lep. , Lym an triidae)popu lat ions[J] . JApp lE nt , 2003, (127): 405~ 412 |
|
[8]
|
祁学忠, 孟小林, 朱应, 等. PCR法证实A cNPV 对小菜蛾的经卵传播[A] . 见: 张芝利, 朴永范, 吴钜文. 中国有害生物综合治理论文集[C], 北京: 中国农业出版社, 1996: 1098~ 1101
|
|
[9]
|
段立清. 昆虫病毒对鳞翅目食叶害虫的亚致死作用[J] . 中国森林病虫, 2002, 21(3) : 33~ 35
|
|
[10]
|
陈新文, 胡志红, V alk JM, 等. 中国棉铃虫单粒包埋核型多角体病毒多角体蛋白基因的序列分析[J] . 中国病毒学, 1997, 12(4): 346~ 353
|
|
[11]
|
席景会, 潘洪玉, 刘伟成, 等. 八字地老虎核型多角体病毒对寄主昆虫繁殖潜势的影响[J] . 中国病毒学, 2000, 15: 76 ~ 79
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