Identification of Oil Tea( Camellia ole ife ra ) Super iorClones by ISSRMolecularMarker

ZHANG Guo-wu; ZHONG Wen-bin; Wuyuntana; TAN Xiao-feng; DU Tian-zhen

ISSR were used to detect the genetic diversity among 10 oil-tea camellia superior clones and 1 controlsamp le ( seedling). 16 p rimers out of 99 were screened out and 135 ISSR markerswere amp lified from these cloneswith 114 polymorphic loci. The average genetic distance between genotypes of cloneswas 0. 419,which was of closerdistance compared with that between superior clones and control samp le, 0. 58. The study showed that there was abig genetic distance between them and established the molecular identification of oil tea superior clones. It meansthat a sound ground was laid down for the molecular breeding of oil-tea.

1. College of Landscape and Art, J iangxi AgriculturalUniversity,Nanchang　330045, J iangxi, China

2. Subtrop ical ExperimentalCenter of Forestry, CAF, Fengyi　336600, J iangxi, China

3. The Key Laboratory of Non-wood Forest Product ofState Forestry Administration, Central South University of Forestry and Technology, Changsha　410004, Hunan, China

Abstract: ISSR were used to detect the genetic diversity among 10 oil-tea camellia superior clones and 1 controlsamp le ( seedling). 16 p rimers out of 99 were screened out and 135 ISSR markerswere amp lified from these cloneswith 114 polymorphic loci. The average genetic distance between genotypes of cloneswas 0. 419,which was of closerdistance compared with that between superior clones and control samp le, 0. 58. The study showed that there was abig genetic distance between them and established the molecular identification of oil tea superior clones. It meansthat a sound ground was laid down for the molecular breeding of oil-tea.

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Reference (20)

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Identification of Oil Tea( Camellia ole ife ra ) Super iorClones by ISSRMolecularMarker

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