RAPD Ana lysis on the Genetic D iversity ofW ild andCultiva ted Popula tion s ofM use lla lasioca rpa

PAN Qing-jie; LI Zheng-hong; WANG Yan; TIAN Jie; GU Yong; LIU Xiu-xian

Random amp lified polymorphic DNA (RAPD ) was amp lified to study the genetic diversity and geneticstructure of Musella lasiocarpa based on twelve wild and cultivated populations. 10 p rimers were screened to use,and 88 bandswere amp lified, among which 85 were polymorphic. At specific level, the percentage of polymorphicbands, the genetic diversity index and Shannon information index were 96. 59% , 0. 289 0 and 0. 441 6. The totalgene differentiation ( Gst) was 0. 568 2, which indicated 43. 13% of genetic variation resided within populationsand slightly higher than that among populations. The genetic identity among populations ranged from 0. 66 to 0. 95.The results from cluster analysis showed that the genetic distance among wild populations was close, and was consistent with their geographical distribution pattern; the genetic distance among cultivated populationswas far and wasnot consistentwith their geographical distribution pattern.

1. Research Institute of Resources Insects, CAF, Kunming　650224, Yunnan, China

2. Research Institute of Forestry, CAF, Beijing　100091, China

Received Date: 2007-02-15

Abstract: Random amp lified polymorphic DNA (RAPD ) was amp lified to study the genetic diversity and geneticstructure of Musella lasiocarpa based on twelve wild and cultivated populations. 10 p rimers were screened to use,and 88 bandswere amp lified, among which 85 were polymorphic. At specific level, the percentage of polymorphicbands, the genetic diversity index and Shannon information index were 96. 59% , 0. 289 0 and 0. 441 6. The totalgene differentiation ( Gst) was 0. 568 2, which indicated 43. 13% of genetic variation resided within populationsand slightly higher than that among populations. The genetic identity among populations ranged from 0. 66 to 0. 95.The results from cluster analysis showed that the genetic distance among wild populations was close, and was consistent with their geographical distribution pattern; the genetic distance among cultivated populationswas far and wasnot consistentwith their geographical distribution pattern.

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Reference (13)

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RAPD Ana lysis on the Genetic D iversity ofW ild andCultiva ted Popula tion s ofM use lla lasioca rpa

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