Studies on Al ka line Solution Extraction of Polysacchar idefrom Sil kworm Pupa and Its Immunomodula ting Activ ities

SUN Long; FENG Ying; HE Zhao; MA Tao; ZHANG Xin

The extraction of polysaccharide from silkworm pupa by alkaline solution was studied. The op timum conditionswere obtained by using orthogonal design L9 (34 ). The result showed the op timum conditions were as follows: concentration of alkaline solution 0. 02 mol·L-1 , temperature 80 ℃, time 3 hours. Under these conditions,the contents of total polysaccharide, p rotein and amino acids in extractiveswere 27. 9% , 38. 1% and 35. 85% respectively. The immunological test in mice showed the polysaccharide of silkworm pupa ( PSP) could increase significantly macrophage phagocytosis, enhance remarkably hemolysin antibody and lymphocyte transformation, whichindicated PSP had evident nonspecific, cellular and humoral immunity.

1. Research Institute of Resources Insects, CAF

2. Key Laboratory of Cultivation and Utilizationof Resource Insects, Sate Forestry Administration, Kunming　650224, Yunnan, China

Received Date: 2007-05-20

Abstract: The extraction of polysaccharide from silkworm pupa by alkaline solution was studied. The op timum conditionswere obtained by using orthogonal design L9 (34 ). The result showed the op timum conditions were as follows: concentration of alkaline solution 0. 02 mol·L-1 , temperature 80 ℃, time 3 hours. Under these conditions,the contents of total polysaccharide, p rotein and amino acids in extractiveswere 27. 9% , 38. 1% and 35. 85% respectively. The immunological test in mice showed the polysaccharide of silkworm pupa ( PSP) could increase significantly macrophage phagocytosis, enhance remarkably hemolysin antibody and lymphocyte transformation, whichindicated PSP had evident nonspecific, cellular and humoral immunity.

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Reference (18)

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Studies on Al ka line Solution Extraction of Polysacchar idefrom Sil kworm Pupa and Its Immunomodula ting Activ ities

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通讯作者: 陈斌, bchen63@163.com

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