Effect of PulsedMagnetic Field on the Growth, Physiolog ica l andBiochem ica l Character istics of P inus m asson iana Ca llus

LIANG Jun; NING Shao-hua; WANG Zhen-chao; ZHANG Xing-yao

Volume 21 Issue 6

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Article Navigation > Forest Research > 2008 > 21(6): 818-824

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Effect of PulsedMagnetic Field on the Growth, Physiolog ica l andBiochem ica l Character istics of P inus m asson iana Ca llus

1.
Research Institute of Forest Ecology, Environment and Protection, CAF, Key Laboratory of Forestry Protection, State Forestry Administration,Beijing　100091, China
2.
Beijing Xishan Experimental Forest Farm, Beijing　100093, China
3.
College of Electronic andInformation Engineering, Hebei University, Baoding　071002, Hebei, China

Received Date: 2008-06-10

Abstract

The present research used Pinus m asson iana callus as the experiment material. P. m assoniana calluswere treated with different pulse rise time and treating time, then the growth condition of P. m assoniana calluswasobserved, the exterior shape, the content of soluble p rotein and soluble sugar and the activity of p rotective enzymesof P. m assoniana calluswere also been discussed. The experiment results were as follows: (1) After treated withpulsed magnetic field, the fresh weight of P. m assoniana callus increased obviously compared with the controlgrouP. The trend of its development was increasing slowly then decreasing sharp ly. ( 2) The treatment of pulsedmagnetic field on the P. m assoniana callus could increase the contents of soluble p rotein and soluble sugar. Andthose parameters increased to the highest level at the pulse rise time of 5 ms and treating time of 8 h. (3) Aftertreated with pulsed magnetic field, the activity of p rotective enzyme system comp rised of peroxidase ( POD ) , superoxide dismutase ( SOD) , catalase (CAT) increased with the imp rovement of pulse rise time and treating time.The activities of POD, SOD, CAT increased to the highest level at the pulse rise time of 5 ms and treating time of8 h and then decreased with different degree. (4) At a certain pulse rise time and treating time, the cellmembraneof P. m assoniana callus could still maintain a relatively strong stability, indicating that cells of P. m asson ianacallus had a certain resistance and endurance against pulsed magnetic field.
- pulsed magnetic field,
- Pinusm assoniana,
- callus,
- growth,
- enzyme activity,
- cellmembrane permeability

References

[1]	张振球. 静电生物效应[M]. 北京:万国学术出版社, 1989: 1 - 5
[2]	Maria P P, Daniele F, Elena P. Senescence delay and change of antioxidant enzyme in Cucum is sativus L. etiolated seedlings by ELFmagnetic fields[J]. Plant Science, 2001, 161 (1) : 45 - 53
[3]	Jae-DukMoon, Hwa-Sook Chung. Acceleration of germination of tomato seed by app lying AC electric and magnetic fields[J]. Journalof Electrostatics, 2000, 48 (2) : 103 - 114
[4]	Yao Yinan, Li Yuan, Yang Yongqing. Effect of seed p retreatment bymagnetic field on the sensitivity of cucumber(Cucum is sativus) seedlings to ultraviolet-B radiation[J]. Environmental and ExperimentalBotany, 2005, 54 (3) : 286 - 294
[5]	夏丽华,郭继勋. 磁场对羊草过氧化物酶的激活效应及同工酶分析[J]. 应用生态学报, 2000, 11 (5) : 699 - 702
[6]	Shin-ichiro H, Yoshimasa I, Kazumasa O. Drastic high magneticfield effect on supp ression of Escherichia coli death [J]. Bioelectrochemistry, 2001, 53 (2) : 149 - 153
[7]	Yoshimasa I, Shin-ichiro H, Kazumasa O. Twelve hours exposure toinhomogeneous high magnetic field after logarithmic growth phase issufficient for drastic supp ression of Escherichia coli death [J].Bioelectrochemisty, 2001, 54 (2) : 101 - 105
[8]	毛宁,黄谚谚. 生物磁技术在农业的应用及机理探讨[J]. 激光生物学报, 1998, 7 (4) : 122 - 126
[9]	Zhang Xiaoyun. Effect and mechanism discuss of magnetic field oncells growth and divide [J]. China Science ( Series B ) , 1989, 2:164 - 170
[10]	赵　剑,马福荣,杨文杰,等. 静电场对苜蓿愈伤组织抗寒能力的影响[J]. 生物物理学报, 2000, 16 (2) : 406 - 412
[11]	石贵玉,周巧劲,郭平生. 高压静电对烟草愈伤组织生长和根分化的效应[J]. 广西植物, 2002, 22 (4) : 364 - 367
[12]	石贵玉,周巧劲,张振球. 高压静电场对银杏愈伤组织生长的影响[J]. 生物物理学报, 1999, 15 (3) : 547 - 550
[13]	石贵玉,邓欢爱,周巧劲. 高压静电场对蟆叶秋海棠愈伤组织生长的效应[J]. 广西科学, 1999, 6 (4) : 296 - 298
[14]	赵亚华. 生物化学试验技术教程[M]. 雅安:四川农大生化教研室, 2000: 210 - 215
[15]	张维杰. 糖复合物生化研究技术(第二版) [M]. 杭州:浙江大学出版社, 1999: 1 - 540
[16]	吴家和,陈志贤,李淑君,等. 棉花体细胞愈伤组织诱导和增殖期间某些代谢产物的动态变化[J]. 中国棉花, 1999, 26 ( 3 ) :17 - 18
[17]	李合生. 植物生理生化试验原理和技术[M]. 北京:高等教育出版社, 2001: 164 - 261
[18]	蒋传英,金承德,吴仁龙,等. 工具酶的活力测定[M]. 上海:上海科学技术出版社, 1982: 36 - 38
[19]	邹　绮. 植物生理学实验指导[M]. 北京: 中国农业出版社, 1995
[20]	郑巧兰,张祖新,赵仲仁. 磁处理对植物生长的影响[J]. 天津农业科学, 1997, 3 (3) : 11 - 13
[21]	弥晓菊,马　越,郭贵云. 磁场处理番茄种子对其生理生化影响的研究[J]. 植物研究, 2000, 19 (1) : 68 - 74

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effect of PulsedMagnetic Field on the Growth, Physiolog ica l andBiochem ica l Character istics of P inus m asson iana Ca llus

1. Research Institute of Forest Ecology, Environment and Protection, CAF, Key Laboratory of Forestry Protection, State Forestry Administration,Beijing　100091, China

2. Beijing Xishan Experimental Forest Farm, Beijing　100093, China

3. College of Electronic andInformation Engineering, Hebei University, Baoding　071002, Hebei, China

Received Date: 2008-06-10

Abstract: The present research used Pinus m asson iana callus as the experiment material. P. m assoniana calluswere treated with different pulse rise time and treating time, then the growth condition of P. m assoniana calluswasobserved, the exterior shape, the content of soluble p rotein and soluble sugar and the activity of p rotective enzymesof P. m assoniana calluswere also been discussed. The experiment results were as follows: (1) After treated withpulsed magnetic field, the fresh weight of P. m assoniana callus increased obviously compared with the controlgrouP. The trend of its development was increasing slowly then decreasing sharp ly. ( 2) The treatment of pulsedmagnetic field on the P. m assoniana callus could increase the contents of soluble p rotein and soluble sugar. Andthose parameters increased to the highest level at the pulse rise time of 5 ms and treating time of 8 h. (3) Aftertreated with pulsed magnetic field, the activity of p rotective enzyme system comp rised of peroxidase ( POD ) , superoxide dismutase ( SOD) , catalase (CAT) increased with the imp rovement of pulse rise time and treating time.The activities of POD, SOD, CAT increased to the highest level at the pulse rise time of 5 ms and treating time of8 h and then decreased with different degree. (4) At a certain pulse rise time and treating time, the cellmembraneof P. m assoniana callus could still maintain a relatively strong stability, indicating that cells of P. m asson ianacallus had a certain resistance and endurance against pulsed magnetic field.

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Reference (21)

[1]	张振球. 静电生物效应[M]. 北京:万国学术出版社, 1989: 1 - 5
[2]	Maria P P, Daniele F, Elena P. Senescence delay and change of antioxidant enzyme in Cucum is sativus L. etiolated seedlings by ELFmagnetic fields[J]. Plant Science, 2001, 161 (1) : 45 - 53
[3]	Jae-DukMoon, Hwa-Sook Chung. Acceleration of germination of tomato seed by app lying AC electric and magnetic fields[J]. Journalof Electrostatics, 2000, 48 (2) : 103 - 114
[4]	Yao Yinan, Li Yuan, Yang Yongqing. Effect of seed p retreatment bymagnetic field on the sensitivity of cucumber(Cucum is sativus) seedlings to ultraviolet-B radiation[J]. Environmental and ExperimentalBotany, 2005, 54 (3) : 286 - 294
[5]	夏丽华,郭继勋. 磁场对羊草过氧化物酶的激活效应及同工酶分析[J]. 应用生态学报, 2000, 11 (5) : 699 - 702
[6]	Shin-ichiro H, Yoshimasa I, Kazumasa O. Drastic high magneticfield effect on supp ression of Escherichia coli death [J]. Bioelectrochemistry, 2001, 53 (2) : 149 - 153
[7]	Yoshimasa I, Shin-ichiro H, Kazumasa O. Twelve hours exposure toinhomogeneous high magnetic field after logarithmic growth phase issufficient for drastic supp ression of Escherichia coli death [J].Bioelectrochemisty, 2001, 54 (2) : 101 - 105
[8]	毛宁,黄谚谚. 生物磁技术在农业的应用及机理探讨[J]. 激光生物学报, 1998, 7 (4) : 122 - 126
[9]	Zhang Xiaoyun. Effect and mechanism discuss of magnetic field oncells growth and divide [J]. China Science ( Series B ) , 1989, 2:164 - 170
[10]	赵　剑,马福荣,杨文杰,等. 静电场对苜蓿愈伤组织抗寒能力的影响[J]. 生物物理学报, 2000, 16 (2) : 406 - 412
[11]	石贵玉,周巧劲,郭平生. 高压静电对烟草愈伤组织生长和根分化的效应[J]. 广西植物, 2002, 22 (4) : 364 - 367
[12]	石贵玉,周巧劲,张振球. 高压静电场对银杏愈伤组织生长的影响[J]. 生物物理学报, 1999, 15 (3) : 547 - 550
[13]	石贵玉,邓欢爱,周巧劲. 高压静电场对蟆叶秋海棠愈伤组织生长的效应[J]. 广西科学, 1999, 6 (4) : 296 - 298
[14]	赵亚华. 生物化学试验技术教程[M]. 雅安:四川农大生化教研室, 2000: 210 - 215
[15]	张维杰. 糖复合物生化研究技术(第二版) [M]. 杭州:浙江大学出版社, 1999: 1 - 540
[16]	吴家和,陈志贤,李淑君,等. 棉花体细胞愈伤组织诱导和增殖期间某些代谢产物的动态变化[J]. 中国棉花, 1999, 26 ( 3 ) :17 - 18
[17]	李合生. 植物生理生化试验原理和技术[M]. 北京:高等教育出版社, 2001: 164 - 261
[18]	蒋传英,金承德,吴仁龙,等. 工具酶的活力测定[M]. 上海:上海科学技术出版社, 1982: 36 - 38
[19]	邹　绮. 植物生理学实验指导[M]. 北京: 中国农业出版社, 1995
[20]	郑巧兰,张祖新,赵仲仁. 磁处理对植物生长的影响[J]. 天津农业科学, 1997, 3 (3) : 11 - 13
[21]	弥晓菊,马　越,郭贵云. 磁场处理番茄种子对其生理生化影响的研究[J]. 植物研究, 2000, 19 (1) : 68 - 74

Effect of PulsedMagnetic Field on the Growth, Physiolog ica l andBiochem ica l Character istics of P inus m asson iana Ca llus

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通讯作者: 陈斌, bchen63@163.com

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Effect of PulsedMagnetic Field on the Growth, Physiolog ica l andBiochem ica l Character istics of P inus m asson iana Ca llus

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