Embroyogen ic Ca llus Induction and Plant Regenera tion ofClones GL9 of Euca lyptus grandis ×E. u rophylla

QIU Zhen-fei; ZENG Bing-shan; LI Xiang-yang; LIU Ying

The embryogenic callus induction and shoot regeneration were studied systematically in elite clone GL9 ofEuca lyptus cultivated widely in south China. We investigated TDZ 0. 02 - 0. 05 mg ·L-1 was the p roperconcentration. The callus induction rate was 92. 9%—100%. When TDZ concentration was higher than 0. 1 mg·L-1, the axillary bud germination was comp letely inhibited. TDZ 0. 02 mg·L-1 with combinations of Cocl2 0. 125mg·L-1 could induct the embryogenic callus. The direct regeneration rate was 13. 39% ±1. 03%, and withcombinations ofNAA 0. 1mg·L-1 could not differentiate directly from callus, but higher regeneration rate (20. 2%±13. 3% ) could be obtained by transferring callus onto regeneration medium. The size of callus can increase to1. 4 fold of its original size in the first subculture in modified MS + TDZ 0. 02 mg·L-1 + NAA 0. 1 mg·L-1medium and the average number of deep2p ink2coloured masses of embryogenic cells on each calluswas 8. 4. In thesecond and third subculture, callus stopped growing further and the number of masses of embryogenic cellsdecreased gradually. Regeneration system could lay a good foundation for further transformation research.

1. Research Institute of Trop ical Foresty, CAF, Guangzhou　510520, Guangdong, China

Received Date: 2008-10-20

Abstract: The embryogenic callus induction and shoot regeneration were studied systematically in elite clone GL9 ofEuca lyptus cultivated widely in south China. We investigated TDZ 0. 02 - 0. 05 mg ·L-1 was the p roperconcentration. The callus induction rate was 92. 9%—100%. When TDZ concentration was higher than 0. 1 mg·L-1, the axillary bud germination was comp letely inhibited. TDZ 0. 02 mg·L-1 with combinations of Cocl2 0. 125mg·L-1 could induct the embryogenic callus. The direct regeneration rate was 13. 39% ±1. 03%, and withcombinations ofNAA 0. 1mg·L-1 could not differentiate directly from callus, but higher regeneration rate (20. 2%±13. 3% ) could be obtained by transferring callus onto regeneration medium. The size of callus can increase to1. 4 fold of its original size in the first subculture in modified MS + TDZ 0. 02 mg·L-1 + NAA 0. 1 mg·L-1medium and the average number of deep2p ink2coloured masses of embryogenic cells on each calluswas 8. 4. In thesecond and third subculture, callus stopped growing further and the number of masses of embryogenic cellsdecreased gradually. Regeneration system could lay a good foundation for further transformation research.

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Reference (10)

[1]	徐华松,徐九龙,黄学林. TDZ在植物组织培养中的作用[J]. 广西植物, 1996, 16 (19) : 77 - 80
[2]	陈云凤,张春荣,黄　霞,等. TDZ对植物体细胞胚胎发生的作用[J]. 植物生理学通讯, 2006, 42 (1) : 127 - 132
[3]	Tibok A, BlackhallNW, Power J B, et al. Op itimized p lant regeneration from callus derived from seeding hypocotyls of Eucalyptus urophylla [J]. Plant Science, 1995 (1101) : 139 - 145
[4]	Greg N, Stephen F C, PhilW, et al. Adventitious Bud Indution inEucalyptus globulus Labill. [J]. Sciety for In Vitro Biology, 2001(37) : 388 - 391
[5]	Luis P B C, Adriane CM G, Silvia B R C, et al. Plant regenerationfrom seedling exp lents of Eucalyptus grandis ×E. urophylla [J].Plant cell, Tissue and organ culture, 1999 (56) : 17 - 23
[6]	卜朝阳. 桉树再生系统的研究[J]. 西南农业学报, 2004, 17(4) : 500 - 503
[7]	欧阳权,曾炼武,李洁汉. 桉树组培育苗新技术[J]. 广西科学,1994, 1 (3) : 49 - 59
[8]	谭德冠,庄南生,黄华孙. 刚果12号桉愈伤组织的诱导与再生植株快繁体系的构建[J]. 热带作物学报, 2005, 26 (3) : 24 - 29
[9]	黄学林,李筱菊,傅家瑞,等. Thidiazuron对苜蓿愈伤组织的乙烯生成及其体细胞胚胎发生的影响[J]. 植物生理学报, 1994, 20(4) : 367 - 372
[10]	欧阳权,彭海忠,李启泉. 桉树愈伤组织发生胚状体研究[J].林业科学, 1981, 17 (1) : 1 - 9

Embroyogen ic Ca llus Induction and Plant Regenera tion ofClones GL9 of Euca lyptus grandis ×E. u rophylla

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References

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通讯作者: 陈斌, bchen63@163.com

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