Molecular Characterization of Elite Genotypes within A First Generation \nBreeding Population of Pinus massoniana Using ISSR

By using 12 selected ISSR (inter-simple sequence repeat)markers, 78 first generation parents from different provenances of Pinus massoniana were assessed to detect their genetic differences. It was found that the percentage of population polymorphic loci, the Neis genetic diversity and the Shannons information diversity index were high, which indicated that the genetic variation of the first generation breeding population was at high level.The genetic distances ranged from 0.115 to 0.776 with an average genetic distance of 0.371. The genetic distance between parents with large latitudinal distance was greater than that with small latitudinal distance. The geographical latitudinal distance between parents of most superior cross combinations was large, and the genetic distance of most parents were greater than 0.430, indicating the parents with both large latitudinal distance and great genetic distance had the largest potential to exhibiting heterosis. It was proposed that elite trees with both large genetic distance and large geographical latitudinal distance should be preferentially considered in the selection of parents and construction of advanced generation breeding population, to maintain high level of genetic variability and improve the genetic gain of breeding.

Abstract: By using 12 selected ISSR (inter-simple sequence repeat)markers, 78 first generation parents from different provenances of Pinus massoniana were assessed to detect their genetic differences. It was found that the percentage of population polymorphic loci, the Neis genetic diversity and the Shannons information diversity index were high, which indicated that the genetic variation of the first generation breeding population was at high level.The genetic distances ranged from 0.115 to 0.776 with an average genetic distance of 0.371. The genetic distance between parents with large latitudinal distance was greater than that with small latitudinal distance. The geographical latitudinal distance between parents of most superior cross combinations was large, and the genetic distance of most parents were greater than 0.430, indicating the parents with both large latitudinal distance and great genetic distance had the largest potential to exhibiting heterosis. It was proposed that elite trees with both large genetic distance and large geographical latitudinal distance should be preferentially considered in the selection of parents and construction of advanced generation breeding population, to maintain high level of genetic variability and improve the genetic gain of breeding.

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Molecular Characterization of Elite Genotypes within A First Generation \nBreeding Population of Pinus massoniana Using ISSR

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通讯作者: 陈斌, bchen63@163.com

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