Studies on Agrobacterium-mediated Transformation of Rose Callus with SeNHX1 Gene

LIU Hui-chao; LIU Meng-gang; GUO Li-juan; JIA Wen-qing

Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus. The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1. Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found. The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5, infection time was 20 min, culture time was 3 days. Adding 100 μmol·L-1 AS, the frequency of transient expression of GUS gene was the highest, which reached about 85% in present study.

1. School of Horticulture Landscape Architecture, He’nan Institute of Science and Technology, Xinxiang 453003, He’nan, China

Abstract: Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus. The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1. Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found. The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5, infection time was 20 min, culture time was 3 days. Adding 100 μmol·L-1 AS, the frequency of transient expression of GUS gene was the highest, which reached about 85% in present study.

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Studies on Agrobacterium-mediated Transformation of Rose Callus with SeNHX1 Gene

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