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Volume 23 Issue 4
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Article Navigation >  Forest Research  > 2010 >  23(4): 626-628

Citation:
shu

Plant Regeneration System of Rare and Endangered Wild Plant Rosa rugosa in Mountain Changbai

  • 1.

    Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin, China

  • 2.

    Forest Farm of Tonghua City, Jilin Province,Tonghua 134001, Jilin, China)

  • The tender stem of Rosa rugosa was used as explant and it suitable medium compositions were screened through uniform design experiments. The results showed that the tissue culture of Rosa rugosa required different kinds of culture medium in different phases. The most suitable culture media were as follows: B5+6-BA 2.90 mg·L-1 for shoots regeneration immediately at base of tender stem, the rate of regeneration was 96%; 1/2 MS(macro-elemenyts)+IBA 0.07 mg·L-1 for rooting, the rooting rate was more than 98.5%. Plant regeneration system of Rosa rugosa had been successfully established.
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  • [1] 汪 松,解 焱. 中国物种红色名录(第一卷)[M]. 北京:高等教育出版社,2004,304-464
    [2] 顾地周,何晓燕,朱俊义,等. 细叶杜香的组织培养和快速繁殖[J]. 植物生理学通讯, 2007,43 (5):898
    [3] 曹孜义,刘国民. 实用植物组织培养技术教程[M].兰州:甘肃科学技术出版社,2002
    [4] 顾地周,朱俊义,姜云天,等. 东北刺人参组培快繁培养基的筛选[J]. 林业科学研究,2008,21(6):867-870
    [5] 顾地周,丛小力,姜云天,等. 色木槭的组织培养与快速繁殖[J]. 植物生理学通讯,2008,44(2):432
    [6] 顾地周,丛小力,宋利丽,等. 木通马兜铃的组织培养和快速繁殖[J]. 植物生理学通讯,2008,44(1):136
    [7] 杨丽娟,顾地周,信天明,等.宽叶杜香叶柄再生体系建立及种质离体保存研究[J].林业科学研究,2010,23(2):293-297
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Plant Regeneration System of Rare and Endangered Wild Plant Rosa rugosa in Mountain Changbai

  • 1. Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin, China
  • 2. Forest Farm of Tonghua City, Jilin Province,Tonghua 134001, Jilin, China)

Abstract: The tender stem of Rosa rugosa was used as explant and it suitable medium compositions were screened through uniform design experiments. The results showed that the tissue culture of Rosa rugosa required different kinds of culture medium in different phases. The most suitable culture media were as follows: B5+6-BA 2.90 mg·L-1 for shoots regeneration immediately at base of tender stem, the rate of regeneration was 96%; 1/2 MS(macro-elemenyts)+IBA 0.07 mg·L-1 for rooting, the rooting rate was more than 98.5%. Plant regeneration system of Rosa rugosa had been successfully established.

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