• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Cloning and Analyzing of Gene PdZFR from Populus deltoides

  • Received Date: 2008-06-13
    Accepted Date: 2010-06-19
  • The full length cDNA and genomic DNA of PdZFR were cloned from Populus deltoides by RACE and Visual Genome Library Walking methods respectively, according to its partial cDNA sequence. Meanwhile, the gene promoter, transcription initiation, intron and exon distribution, spice pattern, the amino acid sequence encoded and the location of this gene on chromosome were analyzed. The tissue or organ-specific expression analysis showed that this gene was related to plant development.
  • 加载中
  • [1]

    Peter K, Klaus FX M, Christian S H. Evaluation and classification of RING-finger domains encoded by the Arabidopsis genome[J].Genome Biology, 2002, 3(4):12-21
    [2]

    Freemont P S .The RING fi nger: a novel protein sequencemotif related to the zinc finger [J]. Ann N Y Acad Sci,1993,68:4174–4192
    [3]

    Jackson P K, Eldridge A G, Freed E,et al. The lore of the RINGs: substrate recognitionand catalysis by ubiquitin ligases [J]. Trends Cell Biol,2000, 10:429-439
    [4]

    Freemont P S.Ubiquitination: RING for destruction[J].Curr Biol,2000,10:R84-R87
    [5]

    Lorick KL, Jensen JP, Fang S. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination [J].Proc Natl Acad Sci USA,1999,96:11364-11369
    [6]

    Dong C H, Agarwal M, Zhang Y Y,et al. The negative regulator of plant cold responses, HOS1,is a RING E3 ligase that mediates the ubiquitinationand degradation of ICE1 [J]. Pans,2006, 103(21): 8281-8286
    [7]

    Yang X, Sun C, Hu Y,et al. Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum[J] J Biosci, 2008, 33:103–112
    [8]

    Schramm G, Bruchhaus I, Roeder T. A simple and reliable 5’-RACE approach[J].Nucleic Acids Res, 2000, 28: e96
    [9]

    Carl W D, Gabriela S. Dveksler PCR Primer: A Laboratory Mannual[M].New york:Cold Spring Harbor Laboratory Press, 2003, 359-389
    [10]

    Bertling W M, Beier F, Reichenberger E. Determination of 5’ ends of specific mRNAs by DNA ligase-dependent amplification [J]. PCR Methods Appl,1993, 3:95-99
    [11]

    Fromont R M, Bertrand E, Pictet R. A highly sensitive method for mapping the 5’ termini of mRNAs,[J]. Nucleic Acids Res, 1993, 21: 1683-1684
    [12]

    Liu X, Gorovsky M A. Mapping the 5’ and 3’ ends of tetrahymena-thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE)[J].Nucleic Acids Res, 1993, 21:4954-4960
    [13]

    Sallie R. Characterization of the extreme 5’ ends of RNA molecules by RNA ligation-PCR[J]. PCR methods Appl,1993, 3:54-56
    [14] 刘 博 ,苏 乔,汤敏谦,等.应用于染色体步移的PCR扩增技术的研究进展 [J].遗传,2006,28 (5):587-595

    [15]

    Peng H, Begg G E, Schultz D C.Reconstitution of the KRAB-KAP-1 repressorcomplex: a model system for defining the molecularanatomy of RING-B box-coiled-coil domain-mediatedprotein-protein interactions [J]. J Mol Biol,2000, 295:1139-1162
    [16]

    Kentsis A, Dwyer E C, Perez J M, et al. The RING domains of the promyelocytic leukemiaprotein PML and the arenaviral protein Z repress translationby directly inhibiting translation initiation factor eIF4E [J].J Mol Biol,2001, 312:609-623
    [17]

    Borden K L. RING domains: master builders of molecular scaffolds [J]. J Mol Biol, 2000, 295:1103-1112
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(3546) PDF downloads(1642) Cited by()

Proportional views

Cloning and Analyzing of Gene PdZFR from Populus deltoides

  • 1. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Abstract: The full length cDNA and genomic DNA of PdZFR were cloned from Populus deltoides by RACE and Visual Genome Library Walking methods respectively, according to its partial cDNA sequence. Meanwhile, the gene promoter, transcription initiation, intron and exon distribution, spice pattern, the amino acid sequence encoded and the location of this gene on chromosome were analyzed. The tissue or organ-specific expression analysis showed that this gene was related to plant development.

Reference (17)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return